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首页> 外文期刊>Surgical Endoscopy >Downregulation of leptin and resistin expression in blood following bariatric surgery.
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Downregulation of leptin and resistin expression in blood following bariatric surgery.

机译：减肥手术后血液中瘦素和抵抗素的表达下调。

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BACKGROUND: Type 2 diabetes (T2D) resolves rapidly after bariatric surgery, even before substantial weight is lost. However, the molecular pathways underlying this phenomenon remain unclear. Microarray data has shown that numerous genes are differentially expressed in blood after bariatric surgery, including resistin and leptin. Resistin and leptin are circulating hormones derived from adipose tissue, which are associated with obesity and insulin resistance. This study examined expression of these genes before and after bariatric surgery in diabetic and nondiabetic obese patients. METHODS: The study included 16 obese patients who underwent bariatric surgery, either Roux-en-Y gastric bypass (RYGB) or adjustable gastric banding. Eight patients had T2D. Preoperative blood samples were collected in PAXgene tubes to stabilize mRNA. Postoperative samples were collected 3 months after surgery. Total RNA was isolated and cDNA was synthesized. Real-time quantitative PCR was used to quantify mRNA. Results were analyzed using Student's t test with a P<0.05 considered significant. RESULTS: Postoperatively, five diabetic patients had discontinued hypoglycemic medications and one showed improved glycemic control. Both leptin and resistin mRNA levels were elevated in the diabetic group but decreased after surgery to levels near those of the nondiabetic group. Greater downregulation of resistin and leptin expression occurred in patients who lost more excess body weight (EBW), while patients who lost less than 10% EBW had a mean increase in expression of the two genes. Downregulation of both genes was more pronounced after RYGB compared to gastric banding. CONCLUSIONS: Downregulation of resistin and leptin gene expression after bariatric surgery may play a role in normalizing obesity-associated insulin resistance. Interestingly, downregulation is greater after RYGB and in patients who lose a greater proportion of EBW. Targeted therapies for obesity and diabetes may be developed by understanding the pathways by which these adipocytokines contribute to obesity and T2D.
机译：背景：2型糖尿病（T2D）在减肥手术后迅速消退，甚至在体重减轻之前也是如此。但是，仍不清楚这种现象的分子途径。微阵列数据显示，减肥手术后血液中许多基因差异表达，包括抵抗素和瘦素。抵抗素和瘦素是源自脂肪组织的循环激素，与肥胖症和胰岛素抵抗有关。这项研究检查了糖尿病和非糖尿病肥胖患者减肥手术前后这些基因的表达。方法：该研究包括16例接受减肥手术的肥胖患者，他们接受了Roux-en-Y胃搭桥术（RYGB）或可调节的胃束带。 8例患者患有T2D。术前血样收集在PAXgene管中以稳定mRNA。术后3个月收集术后样本。分离总RNA并合成cDNA。实时定量PCR用于定量mRNA。使用Student's t检验分析结果，P <0.05被认为是显着的。结果：术后，5例糖尿病患者停用了降糖药物，1例表现出了更好的血糖控制。糖尿病组中，瘦素和抵抗素的mRNA水平均升高，但在手术后降低至接近非糖尿病组的水平。失去更多超重（EBW）的患者发生抵抗素和瘦素表达的更大下调，而失去少于10％EBW的患者这两个基因的表达平均增加。与胃束带相比，RYGB术后两种基因的下调更为明显。结论：减肥手术后抵抗素和瘦素基因表达的下调可能在肥胖相关胰岛素抵抗的正常化中起作用。有趣的是，RYGB后以及失去较大比例EBW的患者下调更大。通过了解这些脂肪细胞因子促成肥胖和T2D的途径，可以开发出针对肥胖和糖尿病的靶向疗法。

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