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首页> 外文期刊>Surgical Endoscopy >Human monocyte activation by biologic and biodegradable meshes in vitro.
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Human monocyte activation by biologic and biodegradable meshes in vitro.

机译：人单核细胞在体外可被生物和可生物降解的网激活。

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BACKGROUND: Inflammation and wound healing play critical roles in the integration of biologic and biodegradable meshes (BMs) at hernia repair sites. Monocytes/macrophages (M/MOs) are key cells controlling inflammation and wound healing. These cells release inflammatory cytokines and growth factors such as interleukin (IL)-1beta, IL-6, IL-8, and vascular endothelial growth factor (VEGF) upon activation. Although BMs have been increasingly used in hernia repairs worldwide, to date, investigations of inflammatory responses to various BMs have been limited. METHODS: Mesh samples of three acellular human dermis-derived biologic meshes (AlloDerm, AlloMax, FlexHD) and one biodegradable synthetic mesh (Bio-A) were placed in 96-well plates. Human peripheral blood mononuclear cells (PBMCs) were isolated from six healthy subjects, added to each well, and incubated for 7 days. Culture supernatants were assayed for IL-1beta, IL-6, IL-8, and VEGF levels using a multiplex bead-base immunoassay system (Bio-Plex). RESULTS: All four meshes induced cytokine expression from activated M/MOs to varying degrees in vitro. FlexHD induced significantly more IL-1beta (2,591 pg/ml) than AlloMax (517 pg/ml), AlloDerm (48 pg/ml), or Bio-A (28 pg/ml) (p < 0.001). AlloMax stimulated a significantly greater quantity of IL-6 (38,343 pg/ml) than FlexHD (19,317 pg/ml), Bio-A (191 pg/ml), or AlloDerm (103 pg/ml) (p < 0.05). Interleukin-8 and VEGF displayed trends similar to that of IL-6. There were no significant differences in cytokine production between AlloDerm and Bio-A. CONCLUSION: This study demonstrated that human macrophages are activated by human dermis-derived biologic and biodegradable meshes in vitro. A wide range of cytokine and growth factor induction was seen among the different mesh products. These differences in M/MO activation may be related to the proprietary processing technologies of the studied meshes. The study results raise the possibility that these differences in M/MO activation could indicate varying intensities of inflammation that control integration of different biologic meshes at the sites of hernia repair.
机译：背景：炎症和伤口愈合在疝修复部位的生物和可降解网片（BMs）的整合中起关键作用。单核细胞/巨噬细胞（M / MOs）是控制炎症和伤口愈合的关键细胞。这些细胞在激活后释放炎性细胞因子和生长因子，例如白介素（IL）-1beta，IL-6，IL-8和血管内皮生长因子（VEGF）。尽管全世界范围内越来越多地将BM用于疝气修复，但对各种BM的炎症反应的研究仍然有限。方法：将三个无细胞人类真皮衍生的生物筛网（AlloDerm，AlloMax，FlexHD）和一个可生物降解的合成筛网（Bio-A）的筛网样品置于96孔板中。从六个健康受试者中分离出人外周血单核细胞（PBMC），将其添加到每个孔中，并孵育7天。使用多重珠基免疫测定系统（Bio-Plex）测定培养上清液的IL-1beta，IL-6，IL-8和VEGF水平。结果：所有四个网片均在不同程度上诱导了激活的M / MOs的细胞因子表达。 FlexHD诱导的IL-1beta（2591 pg / ml）明显高于AlloMax（517 pg / ml），AlloDerm（48 pg / ml）或Bio-A（28 pg / ml）（p <0.001）。与FlexHD（19,317 pg / ml），Bio-A（191 pg / ml）或AlloDerm（103 pg / ml）相比，AlloMax刺激的IL-6（38,343 pg / ml）明显多得多（p <0.05）。白介素8和VEGF显示出与IL-6相似的趋势。 AlloDerm和Bio-A之间的细胞因子产生没有显着差异。结论：这项研究证明人巨噬细胞在体外被人真皮衍生的生物和可生物降解的网状物激活。在不同的网状产品中发现了广泛的细胞因子和生长因子诱导。 M / MO激活中的这些差异可能与所研究网格的专有处理技术有关。研究结果提高了可能性，即M / MO激活的这些差异可能表明炎症强度的变化，从而控制了疝修复部位不同生物网格的整合。

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