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首页> 外文期刊>Surgical Endoscopy >The effect of lavage on intraabdominal cell burden.
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The effect of lavage on intraabdominal cell burden.

机译：灌洗对腹腔内细胞负荷的影响。

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BACKGROUND: Abdominal lavage is a common surgical practice, but few studies have been conducted to assess its efficacy at removing cells from the abdominal cavity, particularly during laparoscopic surgery. METHODS: After three 12-mm trocars were inserted into six female 30-kg pigs at the umbilicus left and right iliac fossae, the abdomen was insufflated with carbon dioxide. The pelvis of each pigs was injected with 6 million radiolabeled LIM 1215 cells. Then the abdominal cavity was irrigated with either 500 ml 0.9% saline, 500 ml 10% betadine solution, or 1 L 0.9% saline. A maximum of 5 L of solution was used for each animal. The lavage fluid was suctioned into separate containers after each aliquot, and each container was measured for radioactivity. RESULTS: Significantly greater numbers of cells were removed by lavage by the first to third lavage cycle; however, after four lavage cycles, relatively few cells were removed by each further cycle. No difference was observed between 500-ml and 1-L aliquots. Additionally, the mechanical efficacy of 0.9% saline and 10% betadine solution appeared similar. CONCLUSION: These findings suggest that optimal lavage consists of four irrigation/suction cycles utilizing 500-ml aliquots.
机译：背景：腹部灌洗是一种常见的外科手术方法，但很少进行研究来评估其从腹腔中去除细胞的功效，特别是在腹腔镜手术期间。方法：将三只12毫米套管针插入六只30千克雌性猪的左右窝的脐部，向腹腔注入二氧化碳。每头猪的骨盆注射600万放射性标记的LIM 1215细胞。然后用500 ml 0.9％盐水，500 ml 10％甜菜碱溶液或1 L 0.9％盐水冲洗腹腔。每只动物最多使用5 L溶液。在每次等分试样之后，将灌洗液吸入单独的容器中，并测量每个容器的放射性。结果：在第一个至第三个灌洗周期中，通过灌洗去除了大量细胞。然而，在四个灌洗循环后，每个后续循环都去除了相对较少的细胞。在500毫升和1升等分试样之间未观察到差异。此外，0.9％盐水和10％甜菜碱溶液的机械功效似乎相似。结论：这些发现表明最佳的灌洗包括使用500 ml等分试样的四个冲洗/抽吸循环。

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