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首页> 外文期刊>Surgical Endoscopy >Effect of acidosis on expression of mesothelial cell plasminogen activator inhibitor type-1.
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Effect of acidosis on expression of mesothelial cell plasminogen activator inhibitor type-1.

机译：酸中毒对间皮细胞纤溶酶原激活物抑制剂1型表达的影响。

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摘要

BACKGROUND: Abdominal mesothelial cells are exposed to carbon dioxide during laparoscopy. Previous data indicate that carbon dioxide increases release and expression of plasminogen activator inhibitor type-1 (PAI-1) and induces acidification. METHODS: To assess the impact resulting from a range of pH, human mesothelial cells were exposed to culturing media balanced to pH levels of 6.0 to 8.0 for 90 min. Samples from cell media were withdrawn at several time points. Concentrations of PAI-1 and PAI-1 activity were measured using enzyme-linked immunoassay techniques. To focus on the effect of clinically relevant pH, cells were subjected to pH 6.4 and 7.4. Samples were withdrawn for PAI-1 assessments and for PAI-1 mRNA analyses. RESULTS: During exposure to various levels of pH, PAI-1 secretion and activity were variable. However, 5 h after exposure, greater concentration and activity of PAI-1 were observed in acidified cultures. More PAI-1 mRNA was isolated after exposure of cells to a pH of 6.4, apparently indicating transcriptional regulation. CONCLUSIONS: Mesothelial cells seem to respond to acidification by an increased release and production of PAI-1 in vitro.
机译：背景：腹腔镜检查过程中腹部间皮细胞暴露于二氧化碳。先前的数据表明，二氧化碳会增加1型纤溶酶原激活物抑制剂（PAI-1）的释放和表达并诱导酸化。方法：为评估一定范围的pH值所产生的影响，将人间皮细胞暴露于pH值为6.0至8.0的培养基中90分钟。在几个时间点从细胞培养基中取出样品。使用酶联免疫测定技术测量PAI-1和PAI-1活性的浓度。为了关注临床相关pH的影响，将细胞置于pH 6.4和7.4。取出样品用于PAI-1评估和PAI-1 mRNA分析。结果：在暴露于各种pH值的过程中，PAI-1的分泌和活性是可变的。然而，暴露后5小时，在酸化的培养物中观察到更高的PAI-1浓度和活性。将细胞暴露于6.4的pH后，分离出更多的PAI-1 mRNA，显然表明转录调控。结论：间皮细胞似乎通过增加PAI-1的体外释放和产生来对酸化作出反应。

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