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首页> 外文期刊>Surgical neurology >Cytological and bacteriological studies of intraoperative autologous blood in neurosurgery.
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Cytological and bacteriological studies of intraoperative autologous blood in neurosurgery.

机译:神经外科手术中自体血的细胞学和细菌学研究。

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摘要

BACKGROUND: To ensure the safety of salvaged blood in neurologic surgery, reinfused blood through the Cell Saver System (CSS) (Hemonetics) was investigated cytologically and bacteriologically. METHODS: Specimens of reinfused blood were cytologically examined with Papanicolaou or Giemsa stains. Reinfused blood and air in the operating theater were investigated by microbiologic techniques. The concentration of dust particles in the theater was determined. RESULTS: Tumor cells were positive in reinfused blood in 5 of 9 specimens with glioblastoma, in 2 of 8 with pituitary adenoma, and 1 of 13 with meningioma. The probability of migration of meningioma cells into reinfused blood was significantly low in comparison with that of glioma cells. Of the 30 specimens studied microbiologically, the bacterial growth was detected in salvaged blood of 14 specimens (46.7%) and in the air of the operating theater for 8 specimens (26.7%). In craniotomy, the contamination rate was 10 of 26 specimens of reinfused blood (38.5%). Most microorganisms were found to be staphylococci. No statistically significant correlation could be found between salvaged blood and air as to contamination or between reinfused blood and the concentration of dust particles in the theater as to bacteriologic results. No infectious complications were found after the operation, though salvaged blood through the CSS was reinfused in 37 patients without glioblastoma or transsphenoidal approach. CONCLUSIONS: The CSS cannot always entrap tumor cells. Salvaged blood should not be reinfused in the patients with glioblastoma or transsphenoidal surgery. None of the patients with reinfusion had any infectious complications. Reinfusion of salvaged blood seems to be safe in neurosurgery.
机译:背景:为确保挽救血液在神经外科手术中的安全性,通过细胞保护系统(CSS)(Hemonetics)对注入的血液进行了细胞学和细菌学研究。方法:用巴氏染色法或吉姆萨染色法对回输的血液标本进行细胞学检查。通过微生物学技术对手术室中重新注入的血液和空气进行了研究。确定剧场中灰尘的浓度。结果:9个胶质母细胞瘤标本中有5​​个,垂体腺瘤8个中有2个,脑膜瘤13个中有1个在回输血液中肿瘤细胞阳性。与神经胶质瘤细胞相比,脑膜瘤细胞迁移到重新注入的血液中的可能性非常低。在微生物学研究的30个标本中,在14个标本(46.7%)的残存血液中和在手术室的空气中检出8个标本(26.7%)的细菌生长。开颅手术中,污染率是26份再灌注血液样本中的10份(38.5%)。发现大多数微生物是葡萄球菌。在残存的空气和空气之间是否存在污染,或者在重新注入的血液与剧院中的尘埃颗粒浓度之间,就细菌学结果而言,没有发现统计学上的显着相关性。手术后未发现感染并发症,尽管在37例没有胶质母细胞瘤或经蝶骨入路的患者中,通过CSS注入了挽救的血液。结论:CSS不能总是包埋肿瘤细胞。胶质母细胞瘤或经蝶窦手术的患者不应再次注入挽救的血液。再灌注患者均无感染并发症。在神经外科手术中,重新注入挽救的血液似乎是安全的。

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