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首页> 外文期刊>Biomaterials >The use of anti-COX2 siRNA coated onto PLGA nanoparticles loading dexamethasone in the treatment of rheumatoid arthritis
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The use of anti-COX2 siRNA coated onto PLGA nanoparticles loading dexamethasone in the treatment of rheumatoid arthritis

机译：负载地塞米松的PLGA纳米颗粒上包被的抗COX2 siRNA在类风湿关节炎中的应用

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In drug delivery systems, some genes have the potential to interrupt unnecessary gene expression in specific target cells. In this study, two types of drug, glucocorticoids and siRNA, were co-delivered into conditioned cells to inhibit the expression of unnecessary genes and proteins involved in arthritis. To deliver the two factors into a human chondrocyte cell line (C28/I2), dexamethasone was first loaded into PLGA nanoparticles, and then drug-loaded PLGA nanoparticles were complexed with poly(ethyleneimine) (PEI)/siRNA. To test the co-delivery of siRNA and dexamethasone into chondrocytes, cells were transfected with green fluorescence protein siRNA (GFP siRNA) and drugs. After transfection with GFP siRNA, 70% reduction of C28/I2 cells demonstrated GFP expression, whereas MOCK carrying PLGA nanoparticles and PLGA nanoparticles without siRNA showed no differences of GFP expressions. COX-2 and iNOS productions in C28/I2 cells were examined after TNF-α pre-treatment to induce expression of arthritis-related molecules in vitro. The reduction of gene and protein expression associated with arthritis by transfection with dexamethasone-loaded and COX-2 siRNA-complexed PLGA nanoparticles was evaluated by RT-PCR, real time-qPCR, immunoblotting, immunohistochemistry, and immunofluorescence imaging.
机译：在药物输送系统中，某些基因有可能中断特定靶细胞中不必要的基因表达。在这项研究中，将两种类型的药物糖皮质激素和siRNA共递送至条件细胞中，以抑制与关节炎有关的不必要基因和蛋白质的表达。为了将这两种因子传递到人软骨细胞系（C28 / I2）中，首先将地塞米松装载到PLGA纳米颗粒中，然后将装载药物的PLGA纳米颗粒与聚（乙烯亚胺）（PEI）/ siRNA复合。为了测试siRNA和地塞米松向软骨细胞的共输送，用绿色荧光蛋白siRNA（GFP siRNA）和药物转染细胞。用GFP siRNA转染后，C28 / I2细胞减少70％显示GFP表达，而带有PLGA纳米颗粒的MOCK和不含siRNA的PLGA纳米颗粒显示GFP表达没有差异。 TNF-α预处理以诱导关节炎相关分子在体外表达后，检查C28 / I2细胞中COX-2和iNOS的产生。通过RT-PCR，实时qPCR，免疫印迹，免疫组织化学和免疫荧光成像评估通过负载地塞米松和COX-2 siRNA复合的PLGA纳米颗粒转染与关节炎相关的基因和蛋白质表达的减少。

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