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首页> 外文期刊>Biomaterials >Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix - based scaffold
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Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix - based scaffold

机译：使用牙囊细胞片结合牙本质基质支架进行牙根再生

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Stem cell mediated tissue engineering has been acknowledged as a prospective strategy for repairing and replacing damaged and lost tissues. However, the low survival rate of implanted stem cells proves to be a major challenge in the management of transplantation failures. While previous studies have indicated the effectiveness of tissue engineered cell sheets in improving the survival rate of implanted cells, we have recently demonstrated the use of treated dentin matrix (TDM) as a biological scaffold and dental follicle cells (DFCs) as the seeding cells for dentinogenesis and tooth root construction. This study proposes a strategy utilizing TDM with human dental follicle cell sheets (DFCSs) for root regeneration. The biological characteristics and changes of human DFCSs under the effect of TDM were studied with scanning electron microscopy, transmission electron microscopy, immunofluorescence microscopy, immunohistochemistry and quantitative real-time PCR. DFCSs combined with TDM were implanted subcutaneously into the dorsum of mice. Histological examination of the harvested grafts revealed a whirlpool-like alignment of the DFCs in multiple layers that were positive for COLI, integrinβ1, fibronectin and alkaline phosphatase (ALP), suggestive of the formation of a rich extracellular matrix. DFCSs, under the effect of TDM, highly expressed DMP-1 and bone sialoprotein (BSP), indicating their potential for odontogenesis and osteogenesis. Importantly,. invivo, TDM could induce and support DFCSs to develop new dentin-pulp like tissues and cementum-periodontal complexes that were positive for markers such as DSP, nestin and VIII factors, COLI and cementum attachment protein (CAP), implying successful root regeneration. Therefore, DFCSs combined with TDM may prove to be a better strategy for the construction of tooth root, and is a prospective approach that could be utilized for the treatment of root or tooth defect or loss in future.
机译：干细胞介导的组织工程已被认为是修复和替换受损和丢失的组织的前瞻性策略。然而，事实证明，植入的干细胞存活率低是应对移植失败的主要挑战。虽然先前的研究表明组织工程化细胞片在提高植入细胞的存活率方面是有效的，但我们最近证明了使用处理过的牙本质基质（TDM）作为生物支架，使用牙囊细胞（DFC）作为接种细胞的方法。牙本质生成和牙根构造。这项研究提出了一种将TDM与人牙囊细胞片（DFCSs）结合用于根再生的策略。通过扫描电子显微镜，透射电子显微镜，免疫荧光显微镜，免疫组织化学和定量实时PCR研究了TDM作用下人DFCS的生物学特性和变化。将与TDM结合的DFCSs皮下植入小鼠的背部。采集的移植物的组织学检查显示，DFC在多层中呈漩涡状排列，这些层对COLI，整联蛋白β1，纤连蛋白和碱性磷酸酶（ALP）呈阳性，提示形成了丰富的细胞外基质。 DFCS在TDM的作用下，高表达DMP-1和骨唾液蛋白（BSP），表明它们具有牙源性和成骨性的潜力。重要的，。在体内，TDM可以诱导和支持DFCSs形成新的牙本质-牙髓样组织和牙骨质-牙周复合体，这些组织对诸如DSP，巢蛋白和VIII因子，COLI和牙骨质附着蛋白（CAP）等标志物呈阳性，这意味着成功的根再生。因此，DFCSs与TDM结合可能是一种更好的牙根构建策略，并且是一种可以在将来用于治疗牙根或牙齿缺损或缺失的前瞻性方法。

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