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首页> 外文期刊>Systems biology in reproductive medicine >17 beta-estradiol prevents experimentally-induced oxidative damage to membrane lipids and nuclear DNA in porcine ovary
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17 beta-estradiol prevents experimentally-induced oxidative damage to membrane lipids and nuclear DNA in porcine ovary

机译：17β-雌二醇可防止实验诱导的猪卵巢膜脂质和核DNA的氧化损伤

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Estrogens, with their principle representative 17-estradiol, contribute to the redox state of cells showing both pro- and antioxidative properties. In the ovary, being the main source of estrogens, maintaining balance between the production and detoxification of ROS is crucial. Whereas ovary estrogen concentration is difficult to estimate, its circulating concentration in women may reach the nanomolar level. The aim of the study was to evaluate the effects of 17-estradiol on oxidative damage to membrane lipids (lipid peroxidation, LPO) and to nuclear DNA in the porcine ovary under basal conditions and in the presence of Fenton reaction (Fe2++H2O2Fe3++(OH)-O-center dot+OH-) substrates. Ovary homogenates and DNA were incubated in the presence of 17-estradiol (1mM-1pM), without/with FeSO4 (30M)+H2O2 (0.5mM). Malondialdehyde+4-hydroxyalkenals (MDA+4-HDA) concentration (LPO index) was measured spectrophotometrically. The concentration of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) (DNA damage index) was measured by HPLC. We observed that 17-estradiol did not alter the basal level of oxidative damage, but reduced Fe2++H2O2-induced oxidative damage to membrane lipids when 10nM and to DNA at concentrations 1nM. In the ovary at near physiological concentration, 17-estradiol prevents experimentally induced oxidative damage. This suggests that under physiological conditions this hormone may contribute to protecting the ovary against oxidative damage.
机译：雌激素，以其主要代表17-雌二醇，有助于细胞的氧化还原状态，同时显示前和抗氧化特性。在卵巢中，作为雌激素的主要来源，保持ROS的产生与解毒之间的平衡至关重要。尽管卵巢雌激素的浓度难以估计，但其在女性体内的循环浓度可能达到纳摩尔水平。该研究的目的是评估17-雌二醇对在基础条件下以及在Fenton反应（Fe2 ++ H2O2Fe3 ++（Fe2 ++ H2O OH）-O-中心点+ OH-）基板。卵巢匀浆和DNA在没有/有FeSO4（30M）+ H2O2（0.5mM）的情况下在17-雌二醇（1mM-1pM）存在下孵育。分光光度法测定丙二醛+ 4-羟基烯醛（MDA + 4-HDA）的浓度（LPO指数）。通过HPLC测量8-氧代-7,8-二氢-2'-脱氧鸟苷（8-oxodG）的浓度（DNA损伤指数）。我们观察到17-雌二醇并没有改变氧化损伤的基础水平，但是当10nM和1nM浓度的DNA降低了Fe2 ++ H2O2诱导的对膜脂质的氧化损伤。在卵巢中，生理浓度接近时，17-雌二醇可防止实验性氧化损伤。这表明在生理条件下，这种激素可能有助于保护卵巢免受氧化损伤。

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