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首页> 外文期刊>Sustainable Humanosphere >Metabolic engineering for prenylated flavonoids in transgenic plants using bacterial andplant prenyltransferases
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Metabolic engineering for prenylated flavonoids in transgenic plants using bacterial andplant prenyltransferases

机译：利用细菌和植物异戊二烯基转移酶对转基因植物中的异戊烯黄酮进行代谢工程

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摘要

Plants produce a large number of secondary metabolites that do not appear to be relevant for primary biological activity. Among these secondary metabolites, polyphenols are common to all plant species and are known as anti-oxidants and UV protectants.Polyphenols modified with prenyl residues have a variety of biological activities, such as anti-tumor, anti-bacterial, anti-virus, anti-oxidant, anti-tyrosinase and estrogenic. Although prenylated polyphenols are attractive natural products because of their various biological activities, their limited occurrence in plants as well as the difficulty of purification from complicated mixtures has limited the potential applications of these compounds for many years; therefore, metabolic engineering with prenyltransferase genes has been of particular interest. The identification of prenyltransferase genes from Streptomyces and S. flavescens enabled us to synthesize prenylated polyphenols via metabolic engineering. In this study, Streptomyces genes {NphB, SCO7190, and NovQ) and S. flavescens genes (N8DT and G6DT) were used for the targeted production of prenylated polyphenols. We modified the subcellular localization of prenyltransferases to express them in the cytosol, plastids, and mitochondria for soluble-type prenyltransferases from Streptomyces (NphB, SCO7190, and NovQ). A total of 624 transgenic L. japonicus were generated and analyzed for the production of prenylated polyphenols, and the effect of modification of the subcellular compartmentation ofprenyltransferases on the production of prenylated polyphenols was evaluated.
机译：植物产生大量的次级代谢产物，这些代谢产物似乎与初级生物学活性无关。在这些次生代谢产物中，多酚是所有植物物种共有的，被称为抗氧化剂和紫外线防护剂。异戊二烯基残基修饰的多酚具有多种生物活性，例如抗肿瘤，抗菌，抗病毒，抗氧化剂，抗酪氨酸酶和雌激素。尽管炔丙基化的多酚由于具有多种生物活性而成为有吸引力的天然产物，但它们在植物中的存在有限以及难以从复杂的混合物中纯化，限制了这些化合物多年的潜在应用。因此，具有异戊二烯基转移酶基因的代谢工程特别受到关注。从链霉菌和苦参中异戊二烯转移酶基因的鉴定使我们能够通过代谢工程合成异戊烯基化多酚。在这项研究中，链霉菌基因（NphB，SCO7190和NovQ）和苦参链球菌基因（N8DT和G6DT）被用于目标化的早烷基化多酚。我们修改了异戊二烯基转移酶的亚细胞定位，以在链霉菌（NphB，SCO7190和NovQ）的可溶性型异戊二烯基转移酶的胞质，质体和线粒体中表达它们。总共产生了624个转基因日本刺参并分析了异戊烯基化多酚的产生，并评估了异戊二烯基转移酶的亚细胞区隔修饰对异戊二烯化多酚的产生的影响。

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