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首页> 外文期刊>Parasitology >A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples.
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A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples.

机译：一种基于PCR的新方法，可特异性扩增来自人类血样中不同血吸虫物种的DNA。

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摘要

Currently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific and sensitive diagnostic methods, mainly based on the polymerase chain reaction (PCR) technique. Nevertheless, these have been only applied for the diagnosis of 1 out of 4 Schistosoma species affecting man (S. mansoni). Additionally, application of specific PCR has been exclusively used for blood or faecal patients' samples. Here, we develop a new, high sensitive PCR approach that allows the genus- and species-specific amplification of the main 4 Schistosoma species causing disease in man plus S. bovis. We further successfully apply this technique for the detection of parasite DNA in easy-to-handle urine samples from patients with schistosomiasis. With these samples, we have found 94.4% sensitivity and 99.9% specificity when applying a genus-specific (Schistosoma spp.) primer pair, and 100% sensitivity and 98.9% specificity in a species-specific (S. mansoni) PCR.
机译：目前可用于诊断人类血吸虫病的方法通常缺乏足够的敏感性和特异性。最近，主要基于聚合酶链反应（PCR）技术的几位作者已经开发出了更具体，更灵敏的诊断方法。然而，这些仅用于诊断影响人（S. mansoni）的4种血吸虫物种中的1种。此外，特异性PCR的应用已专门用于血液或粪便患者的样品。在这里，我们开发了一种新的，高灵敏度的PCR方法，该方法允许对引起人类加牛链球菌疾病的4种主要血吸虫物种进行属和种特异性扩增。我们进一步成功地将该技术用于血吸虫病患者易于处理的尿液样品中寄生虫DNA的检测。对于这些样品，我们发现在应用属特异性（Schistosoma spp。）引物对时，灵敏度为94.4％，特异性为99.9％;在物种特异性（mansoni）PCR中，灵敏度为100％，特异性为98.9％。

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