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首页> 外文期刊>Parasitology >Antiparasitic effect of calcium and magnesium ion-free buffer treatments against a common monogenean Neobenedenia girellae.
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Antiparasitic effect of calcium and magnesium ion-free buffer treatments against a common monogenean Neobenedenia girellae.

机译：不含钙和镁离子的缓冲液处理对普通单基因新鞭毛虫的抗寄生虫作用。

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摘要

This study investigated a new effective method for controlling the capsalid monogenean Neobenedenia girellae. We examined in vitro and in vivo the effect on the percentage survival of N. girellae in buffers containing different metallic ions. Decreased survival was observed in buffer solutions lacking two ions. In particular, the percentage survival of N. girellae was significantly decreased after 10 min exposure to buffer containing neither Ca(2+) nor Mg(2+). Transmission electron microscopic observations showed that treatment with this buffer disrupted intercellular junctions. This significant effect on percentage survival of N. girellae using Ca(2+)/Mg(2+)-free buffer was confirmed in an in vivo assay. Ca(2+)/Mg(2+)-free buffer had no effect on the condition of the host, spotted halibut Verasper variegates (Pleuronectidae). These results suggest that treatment with Ca(2+)/Mg(2+)-free buffer is a new effective control method, which could replace existing control methods.
机译：这项研究调查了一种新的有效方法来控制衣壳单基因新本尼德尼亚鞭毛虫。我们在体外和体内研究了在含有不同金属离子的缓冲液中，对长颈椎猪笼草存活率的影响。在缺少两个离子的缓冲溶液中观察到存活率降低。特别是，暴露于既不含Ca（2+）也不含Mg（2+）的缓冲液10分钟后，长鼻猪笼草的存活百分比显着降低。透射电子显微镜观察表明，用该缓冲液处理破坏了细胞间连接。在体内实验中证实了使用无Ca（2 +）/ Mg（2+）的缓冲液对长鼻猪笼草存活率的显着影响。不含Ca（2 +）/ Mg（2+）的缓冲液对宿主的状况无影响，斑点大比目鱼Verasper variegates（Pleuronectidae）。这些结果表明用无Ca（2 +）/ Mg（2+）的缓冲液处理是一种新的有效控制方法，它可以替代现有的控制方法。

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