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首页> 外文期刊>Parasitology >Differential N-glycan- and protein-directed immune responses in Dictyocaulus viviparus-infected and vaccinated calves.
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Differential N-glycan- and protein-directed immune responses in Dictyocaulus viviparus-infected and vaccinated calves.

机译：细支ic（Dictyocaulus viviparus）感染和接种的犊牛的N-聚糖和蛋白质定向免疫反应不同。

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Calves with naturally acquired Dictyocaulus viviparus infection mount an effective immune response. In the search for protection-inducing antigens, we found that several D. viviparus third-stage larval (L3) and adult ES products carry N-glycans. Deglycosylation of the worm antigens using PNGase F resulted in reduced IgA, IgE, IgG1 and IgG2 (but not IgM) reactivities in sera of primary infected animals, suggesting that the carbohydrate moieties contained immunodominant epitopes. Challenge infection resulted in increased specific serum antibody levels against ES and L3 in the re-infected and challenge control groups. Testing of sera by enzyme-linked immunosorbent assay (ELISA) demonstrated a significant increase in IgG1 and IgE (but not IgA or IgG2) reactivity against the deglycosylated antigens in the re-infected group compared with the challenge control group. Sera from calves vaccinated with irradiated larvae showed a strong anti-N-glycan response, but no booster response against the protein backbone after challenge infection, consistent with the absence of a memory response. Together, our results suggest that D. viviparus proteins carry immunodominant N-glycan moieties that elicit a strong but short-lived immune response during infection and after vaccination, whereas the protein backbones effectively induce a memory response which results in a long-lasting, potentially protective immune response in re-infected, but not in vaccinated calves.
机译：患有自然获得的Dictyocaulus viviparus感染的犊牛会产生有效的免疫反应。在寻找诱导保护性抗原的过程中，我们发现几种D. viviparus幼虫（L3）和成年ES产品带有N-聚糖。使用PNGase F对蠕虫抗原进行去糖基化会导致原发感染动物血清中的IgA，IgE，IgG1和IgG2（而非IgM）反应性降低，这表明碳水化合物部分含有免疫优势表位。挑战感染导致重新感染和挑战对照组中针对ES和L3的特异性血清抗体水平增加。通过酶联免疫吸附测定（ELISA）进行的血清测试表明，与感染对照组相比，再感染组中IgG1和IgE（而非IgA或IgG2）对脱糖基化抗原的反应性显着提高。接种感染幼虫的牛犊血清显示出强烈的抗N-聚糖反应，但在激发感染后没有针对蛋白质主链的加强反应，这与记忆反应的缺乏一致。在一起，我们的结果表明，D。viviparus蛋白带有免疫优势的N-聚糖部分，在感染过程中和接种疫苗后会引发强烈但短暂的免疫反应，而蛋白质骨架有效诱导记忆反应，从而导致持久的，潜在的保护性免疫反应在重新感染的牛中，但不在接种牛的牛中。

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