• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >A novel coagulation inhibitor from Schistosoma japonicum.
【24h】

A novel coagulation inhibitor from Schistosoma japonicum.

机译：日本血吸虫的新型凝血抑制剂。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Little is known about the molecular mechanisms whereby the human blood fluke Schistosoma japonicum is able to survive in the host venous blood system. Protease inhibitors are likely released by the parasite enabling it to avoid attack by host proteolytic enzymes and coagulation factors. Interrogation of the S. japonicum genomic sequence identified a gene, SjKI-1, homologous to that encoding a single domain Kunitz protein (Sjp_0020270) which we expressed in recombinant form in Escherichia coli and purified. SjKI-1 is highly transcribed in adult worms and eggs but its expression was very low in cercariae and schistosomula. In situ immunolocalization with anti-SjKI-1 rabbit antibodies showed the protein was present in eggs trapped in the infected mouse intestinal wall. In functional assays, SjKI-1 inhibited trypsin in the picomolar range and chymotrypsin, neutrophil elastase, FXa and plasma kallikrein in the nanomolar range. Furthermore, SjKI-1, at a concentration of 7·5 μ m, prolonged 2-fold activated partial thromboplastin time of human blood coagulation. We also demonstrate that SjKI-1 has the ability to bind Ca++. We present, therefore, characterization of the first Kunitz protein from S. japonicum which we show has an anti-coagulant properties. In addition, its inhibition of neutrophil elastase indicates SjKI-1 have an anti-inflammatory role. Having anti-thrombotic properties, SjKI-1 may point the way towards novel treatment for hemostatic disorders.
机译：关于人类血吸虫日本血吸虫能够在宿主静脉血液系统中存活的分子机制知之甚少。蛋白酶抑制剂很可能被寄生虫释放，从而使其能够避免宿主蛋白水解酶和凝血因子的攻击。对日本血吸虫基因组序列的询问确定了一个基因SjKI-1，该基因与编码单结构域Kunitz蛋白（Sjp_0020270）的基因同源，我们在大肠杆菌中以重组形式表达并纯化了该基因。 SjKI-1在成虫和卵中高度转录，但其在尾c和血吸虫中的表达非常低。用抗SjKI-1兔抗体进行的原位免疫定位显示该蛋白存在于感染小鼠小肠壁中捕获的卵中。在功能测定中，SjKI-1在皮摩尔范围内抑制胰蛋白酶，而在纳摩尔范围内抑制胰凝乳蛋白酶，嗜中性弹性蛋白酶，FXa和血浆激肽释放酶。此外，浓度为7·5μm的SjKI-1可延长人体凝血的2倍活化部分凝血活酶时间。我们还证明了SjKI-1具有结合Ca ++的能力。因此，我们介绍了日本血吸虫的第一个Kunitz蛋白的特征，我们证明它具有抗凝血特性。另外，其对嗜中性粒细胞弹性蛋白酶的抑制表明SjKI-1具有抗炎作用。具有抗血栓形成特性，SjKI-1可能为止血病的新疗法指明道路。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号