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首页> 外文期刊>Parasitology >Colonization of Rhodnius prolixus gut by Trypanosoma cruzi involves an extensive parasite killing
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Colonization of Rhodnius prolixus gut by Trypanosoma cruzi involves an extensive parasite killing

机译：克鲁氏锥虫对罗氏螺旋体肠道的定殖涉及广泛的寄生虫杀死

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Trypanosoma cruzi, the etiological agent of Chagas disease, is ingested by triatomines during their bloodmeal on an infected mammal. Aiming to investigate the development and differentiation of T. cruzi inside the intestinal tract of Rhodnius prolixus at the beginning of infection we fed insects with cultured epimastigotes and blood trypomastigotes from infected mice to determine the amount of recovered parasites after ingestion. Approximately 20% of the ingested parasites was found in the insect anterior midgut (AM) 3 h after feeding. Interestingly, a significant reduction (80%) in the numbers of trypomastigotes was observed after 24 h of infection suggesting that parasites were killed in the AM. Moreover, few parasites were found in that intestinal portion after 96 h of infection. The evaluation of the numbers of parasites in the posterior midgut (PM) at the same periods showed a reduced parasite load, indicating that parasites were not moving from the AM. Additionally, incubation of blood trypomastigotes with extracts from R. prolixus AMs revealed that components of this tissue could induce significant death of T. cruzi. Finally, we observed that differentiation from trypomastigotes to epimastigotes is not completed in the AM; instead we suggest that trypomastigotes change to intermediary forms before their migration to the PM, where differentiation to epimastigotes takes place. The present work clarifies controversial points concerning T. cruzi development in insect vector, showing that parasite suffers a drastic decrease in population size before epimastigonesis accomplishment in PM.
机译：恰加斯氏锥虫病是恰加斯氏病的病原体，它在被感染的哺乳动物的血粉中被三松果碱摄入。为了在感染开始时调查罗氏螺旋体肠道内克氏锥虫的发育和分化，我们向昆虫喂食了感染的小鼠的培养的鞭毛鞭毛虫和血色鞭毛鞭毛虫，以确定摄入后回收的寄生虫数量。喂食3小时后，在昆虫的前中肠（AM）中发现了约20％的摄入的寄生虫。有趣的是，感染24小时后，观察到的锥虫的数量显着减少（80％），表明寄生虫在AM中被杀死。此外，感染96小时后，在该肠道部分几乎没有发现寄生虫。对同一时期后中肠（PM）中的寄生虫数量进行评估显示，寄生虫负荷降低了，这表明寄生虫并未从AM中移出。此外，将血锥鞭毛体与来自Prolixus AMs的提取物一起温育发现，该组织的成分可能会导致克鲁维氏锥虫的严重死亡。最后，我们观察到在AM中尚未完成从拟鞭毛体到副鞭毛体的分化。取而代之的是，我们建议锥虫在转移到PM之前先转变为中间形式，然后再分化成PM。目前的工作澄清了有关昆虫媒介中克鲁氏锥虫发育的争议点，表明寄生虫在PM发生mas鞭毛病之前遭受种群数量的急剧减少。

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