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首页> 外文期刊>Parasitology >The TcTASV proteins are novel promising antigens to detect active Trypanosoma cruzi infection in dogs
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The TcTASV proteins are novel promising antigens to detect active Trypanosoma cruzi infection in dogs

机译：TcTASV蛋白是新型有前途的抗原，可检测犬的活动性克鲁氏锥虫感染

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摘要

In regions where Chagas disease is endemic, canine Trypanosoma cruzi infection is highly correlated with the risk of transmission of the parasite to humans. Herein we evaluated the novel TcTASV protein family (subfamilies A, B, C), differentially expressed in bloodstream trypomastigotes, for the detection of naturally infected dogs. A gene of each TcTASV subfamily was cloned and expressed. Indirect enzyme-linked immunosorbent assays (ELISA) were developed using recombinant antigens individually or mixed together. Our results showed that dogs with active T. cruzi infection differentially reacted against the TcTASV-C subfamily. The use of both TcTASV-C plus TcTASV-A proteins (Mix A+C-ELISA) enhanced the reactivity of sera from dogs with active infection, detecting 94% of the evaluated samples. These findings agree with our previous observations, where the infected animals exhibited a quick anti-TcTASV-C antibody response, coincident with the beginning of parasitaemia, in a murine model of the disease. Results obtained in the present work prove that the Mix A+C-ELISA is a specific, simple and cheap technique to be applied in endemic areas in screening studies. The Mix A+C-ELISA could help to differentially detect canine hosts with active infection and therefore with high impact in the risk of transmission to humans.
机译：在恰加斯病流行的地区，犬克氏锥虫感染与将寄生虫传播给人类的风险高度相关。在这里，我们评估了新型TcTASV蛋白家族（亚家族A，B，C），该蛋白在血锥虫中有差异表达，用于检测自然感染的狗。克隆并表达每个TcTASV亚家族的基因。使用重组抗原单独或混合在一起开发了间接酶联免疫吸附测定（ELISA）。我们的研究结果表明，患有活动性克氏锥虫感染的狗对TcTASV-C亚家族的反应不同。同时使用TcTASV-C和TcTASV-A蛋白（Mix A + C-ELISA）可增强患有活动性感染犬的血清反应性，可检测94％的评估样品。这些发现与我们以前的观察结果一致，在该疾病的小鼠模型中，被感染的动物表现出快速的抗TcTASV-C抗体反应，与寄生虫血症的发生相吻合。在本工作中获得的结果证明，Mix A + C-ELISA是一种特定，简单且便宜的技术，可用于筛查研究中的流行地区。混合A + C-ELISA可以帮助差异检测具有主动感染的犬宿主，因此对传播给人类的风险具有重大影响。

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