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首页> 外文期刊>Parasitology >A trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks in Kagoshima Prefecture, Japan.
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A trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks in Kagoshima Prefecture, Japan.

机译：从日本鹿儿岛县自然感染的嗜血血ema中分离出的一种锥虫物种。

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摘要

Common arthropod vectors for trypanosomes are flies, fleas and bugs. This study reports on an unknown trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks, hereby, referred to as Trypanosoma KG1 isolate. The parasite has been successfully cultured in vitro with L929 or HEK 293T cell line as feeder cells. This trypanosome cannot survive in vitro without feeder cells. Following experimental infections of ticks, the trypomastigote-like and the epimastigote-like forms of this trypanosome could be detected by Giemsa-stained smears of the midgut and salivary glands of Ornithodoros moubata ticks which were made to feed on a culturing medium containing Trypanosoma KG1 isolate through an artificial membrane. Trypanosoma KG1 isolate could also be detected from Giemsa-stained smears of the haemolymph up to 30 days post-inoculation into the O. moubata haemocoel. Trypanosoma KG1 isolate cannot be propagated in laboratory animals including mice, rats, rabbits and sheep. A phylogenetic tree constructed with the 18S rRNA gene indicates that Trypanosoma KG1 is a member of the stercorarian trypanosomes.
机译：锥虫的常见节肢动物载体是苍蝇，跳蚤和虫子。这项研究报告了从自然感染的嗜血血Ha中分离出的一种未知的锥虫物种，在此称为锥虫KG1分离株。该寄生虫已成功地在体外用L929或HEK 293T细胞系作为饲养细胞进行培养。没有饲养细胞，这种锥虫无法在体外存活。在对experimental进行实验性感染之后，可以通过吉姆萨染色的鸟粪中肠和唾液腺的吉姆萨染色涂片来检测这种锥虫的锥虫类和副鞭毛体状形式，然后以含有锥虫KG1分离株的培养基为食通过人造膜。锥虫KG1分离株也可在吉姆萨染色的血淋巴涂片中检测到，直至接种O. moubata血细胞后30天。锥虫KG1分离株不能在包括小鼠，大鼠，兔和绵羊在内的实验动物中繁殖。用18S rRNA基因构建的系统树表明锥虫KG1是甾体锥虫的成员。

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