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首页> 外文期刊>Parasitology >Quantitative analysis of parasite DNA in the blood of immunized and naive mice after infection with Neospora caninum.
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Quantitative analysis of parasite DNA in the blood of immunized and naive mice after infection with Neospora caninum.

机译：犬新孢子虫感染后免疫和幼稚小鼠血液中寄生虫DNA的定量分析。

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Real-time PCR was used to study the duration and level of parasitaemia in mice immunized with immune-stimulating complexes (iscoms) containing recombinant NcSRS2, one of the immunodominant surface antigens of Neospora caninum. After challenge infection, blood was collected daily for 9 days. During this period the amounts of parasite DNA detected in immunized mice were significantly lower (P<0.001), and the duration of parasitaemia appeared to be shorter, than in non-immunized controls. Furthermore, the degree of parasitaemia seemed to correlate well with the amount of N. caninum DNA in the brain 3 weeks post-inoculation and with disease severity measured as changes in body weight. These results indicate that the protective immunity induced by the NcSRS2-iscoms was sufficient to reduce the level of parasitaemia, which probably reduced the number of parasites reaching the brain, and could be the reason for the reduction in brain parasite load and clinical symptoms. Furthermore, real-time PCR was found tobe a sensitive means for rapid assessment of N. caninum in blood.
机译：实时PCR用于研究用含有重组NcSRS2（犬新孢子虫的免疫优势表面抗原之一）的免疫刺激复合物（iscoms）免疫的小鼠的寄生虫血症的持续时间和水平。激发感染后，每天采血9天。在此期间，与未免疫的对照组相比，免疫小鼠中检出的寄生虫DNA量显着降低（P <0.001），寄生虫血症的持续时间似乎更短。此外，寄生虫血症的程度似乎与接种后3周脑中犬新孢子虫DNA的含量以及以体重变化衡量的疾病严重程度密切相关。这些结果表明，NcSRS2-iscoms诱导的保护性免疫足以降低寄生虫血症的水平，这可能减少了到达大脑的寄生虫的数量，并且可能是减少脑部寄生虫负荷和临床症状的原因。此外，发现实时PCR是快速评估血液中犬新孢子虫的灵敏手段。

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