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首页> 外文期刊>Parasitology >Does Litomosoides sigmodontis synthesize dimethylethanolamine from choline ?
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Does Litomosoides sigmodontis synthesize dimethylethanolamine from choline ?

机译：Litomosoides sigmodontis是否从胆碱合成二甲基乙醇胺？

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摘要

Juvenile female Litomosoides sigmodontis secrete a protein (Juv-pl20) highly modified with dimethylethanolamine (DMAE). In an attempt to establish the source of this decoration worms were pulsed with [~3H]-choline and [~3H]-ethanolamine and the radio-isotope labelled products analysed. Both isotope labels were successfully taken up by the worms, as demonstrated by labelling of phospholipids with [~3H]-choline, being predominantly incorporated into phos-phatidylcholine and [~3H]-ethanolamine into phosphatidylethanolamine. Isotope labelling of phosphatidylethanolamine was particularly striking with the worms taking up ~30 times as much labelled ethanolamine as choline. It was possible to detect faint labelling of Juv-p120 with [~3H]-ethanolamine after prolonged exposure periods but, unlike the situation with the phospholipids, it was much more readily labelled with [~3H]-choline. When pulsing with [~3H]-ethanolamine it was also possible to detect isotope-labelled phosphatidylcholine, which may ultimately account for the low levels of labelling of Juv-pl20. Overall our results raise the previously unconsidered but intriguing possibility that in L. sigmodontis, choline may be the precursor of DMAE.
机译：少年雌性类鳞癣分泌一种蛋白质（Juv-p120），该蛋白质经二甲基乙醇胺（DMAE）高度修饰。为了确定这种装饰物的来源，用[〜3H]-胆碱和[〜3H]-乙醇胺对蠕虫进行脉冲处理，并对放射性同位素标记的产物进行分析。蠕虫成功地吸收了这两种同位素标记，正如用[〜3H]-胆碱标记磷脂所证明的那样，它们主要掺入了磷脂酰胆碱和[〜3H]-乙醇胺中并掺入了磷脂酰乙醇胺中。磷脂酰乙醇胺的同位素标记特别引人注目，蠕虫所吸收的乙醇胺含量约为胆碱的30倍。延长暴露时间后，可以用[〜3H]-乙醇胺检测到Juv-p120的微弱标记，但与磷脂的情况不同，它更容易用[〜3H]-胆碱标记。当用[〜3H]-乙醇胺脉冲时，还可能检测到同位素标记的磷脂酰胆碱，这可能最终解释了Juv-p120的低标记水平。总的来说，我们的研究结果提出了以前未曾考虑过但有趣的可能性，即在胆囊肿中，胆碱可能是DMAE的前身。

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