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首页> 外文期刊>Parasitology >Cytokine expression in dogs with natural Leishmania infantum infection.
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Cytokine expression in dogs with natural Leishmania infantum infection.

机译：天然利什曼原虫婴儿感染犬的细胞因子表达。

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摘要

The aim of this study was to evaluate cytokine expression in 22 Leishmania infantum naturally infected dogs, in order to correlate this parameter with the clinical status of infected animals. After 4 and 8 months from the first diagnosis of Leishmania infection, clinical and laboratory examination of dogs was performed and peripheral blood mononuclear cells (PBMC) were isolated. The cytokine profile was analysed in terms of IFN-gamma, IL-4, IL-10 and TNF-alpha mRNA expression in cultured PBMC by a semi-quantitative reverse transcriptase-PCR. Thirteen out of 22 Leishmania-infected dogs remained asymptomatic in the follow-up, while 9 showed clinical signs of leishmaniasis. IL-4, IL-10, TNF-alpha and IFN-gamma mRNA levels were not significantly different in asymptomatic compared to symptomatic animals 4 months from the diagnosis of Leishmania infection, but were significantly higher in symptomatic versus asymptomatic dogs after 8 months from diagnosis. In addition, IL-4, IL-10 and TNF-alpha mRNA levels significantly increased only in symptomatic dogs at 8 months, in comparison to the levels found at 4 months. These results show a mixed Th1 and Th2 cytokine response in Leishmania-infected dogs, with higher cytokine expression in dogs with manifest clinical disease, during the second follow-up after 8 months from the first diagnosis of infection.
机译：这项研究的目的是评估22只天然感染利什曼原虫的狗中细胞因子的表达，以便将该参数与被感染动物的临床状况相关联。从首次诊断利什曼原虫感染起4和8个月后，对狗进行了临床和实验室检查，并分离了外周血单个核细胞（PBMC）。通过半定量逆转录酶-PCR，以培养的PBMC中的IFN-γ，IL-4，IL-10和TNF-αmRNA表达来分析细胞因子谱。在随访中，有22只感染利什曼原虫的狗中有13只保持无症状，而9只表现出利什曼病的临床体征。在诊断为利什曼原虫感染后4个月，无症状动物与有症状动物相比，IL-4，IL-10，TNF-α和IFN-γmRNA水平无显着差异，但在确诊8个月后，有症状和无症状的狗中IL-4，IL-10，TNF-α和IFN-γmRNA水平无明显差异。此外，与有症状的狗的4个月时相比，仅在有症状的狗中的IL-4，IL-10和TNF-αmRNA水平在第8个月时才显着增加。这些结果表明，在首次确诊感染后的8个月内进行第二次随访期间，利什曼原虫感染犬的Th1和Th2细胞因子反应混合，在患有明显临床疾病的犬中细胞因子表达较高。

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