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首页> 外文期刊>Parasitology >Identification of the agent causing visceral leishmaniasis in Uzbeki and Tajiki foci by analysing parasite DNA extracted from patients' Giemsa-stained tissue preparations.
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Identification of the agent causing visceral leishmaniasis in Uzbeki and Tajiki foci by analysing parasite DNA extracted from patients' Giemsa-stained tissue preparations.

机译：通过分析从患者吉姆萨染色组织制剂中提取的寄生虫DNA，鉴定导致乌兹别克和塔吉克病灶内脏利什曼病的病原体。

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摘要

Our present study is the first attempt to characterize Leishmania parasites from foci in Uzbekistan and Tajikistan endemic for visceral leishmaniasis (VL). PCR-sequencing of the ribosomal internal transcribed spacer 1 and multilocus microsatellite typing (MLMT) were applied to DNA extracted from preparations of Giemsa-stained bone marrow aspirates from 13 cases of VL. L. infantum was shown to cause VL currently occurring in this area. MLMT applying 14 microsatellite markers, previously shown to be polymorphic for strains of the L. donovani complex, revealed that microsatellite profiles of parasites causing human VL in the Namangan and Jizzakh regions in Uzbekistan, and Penjikent region in Tajikistan, basically coincide with those of strains of L. infantum MON-1. Furthermore, these parasites were assigned to a distinct cluster genetically clearly separated from the populations of L. infantum MON-1 from Europe, the Middle East and North Africa. The existence of a genetically homogeneous but distinct group of L. infantum MON-1 indicates that the parasites circulating in the Uzbeki and Tajiki foci studied have been restricted there for a long time rather than having been recently introduced from elsewhere by human or animal reservoir migration.
机译：我们目前的研究是首次尝试从乌兹别克斯坦和塔吉克斯坦的内脏利什曼病（VL）流行病灶中鉴定利什曼原虫的寄生虫。核糖体内部转录间隔子1的PCR测序和多位点微卫星分型（MLMT）用于从13例VL的Giemsa染色的骨髓抽吸物制剂中提取的DNA。已表明婴儿乳杆菌可导致该区域目前发生的VL。 MLMT应用了14种微卫星标记，以前显示出它们对多诺氏乳杆菌（L. donovani）复合体具有多态性，它揭示了在乌兹别克斯坦的Namangan和Jizzakh地区以及塔吉克斯坦的Penjikent地区引起人类VL的寄生虫的微卫星概况，与这些菌株基本一致婴儿乳杆菌MON-1。此外，这些寄生虫被分配到一个与欧洲，中东和北非的婴儿乳杆菌MON-1种群在遗传上明显分开的独特簇。遗传上同质但独特的婴儿乳球菌MON-1的存在表明，研究的乌兹别克和塔吉克疫源地中传播的寄生虫已被限制很长一段时间，而不是最近由于人类或动物的水库迁移而从其他地方引入。

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