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首页> 外文期刊>Parasitology >Identification and biochemical characterization of two novel peroxiredoxins in a liver fluke, Clonorchis sinensis.
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Identification and biochemical characterization of two novel peroxiredoxins in a liver fluke, Clonorchis sinensis.

机译：肝吸虫中的两种新型过氧化物酶的鉴定和生化特性。

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SUMMARYWe identified 2 novel genes encoding different 2-Cys peroxiredoxins (PRxs), designated CsPRx2 and CsPRx3, in Clonorchis sinensis, which invades the human hepatobiliary tracts. The CsPRx2 gene expression was temporally increased along with the parasite's development and its protein product was detected in almost all parts of adult worms including subtegument, as well as excretory-secretory products. Conversely, CsPRx3 expression was temporally maintained at a basal level and largely restricted within interior parts of various tissues/organs. The recombinant forms of CsPRx proteins exhibited reducing activity against various hydroperoxides in the presence of either thioredoxin or glutathione (GSH) as a reducing equivalent, although they preferred H2O2 and GSH as a catalytic substrate and electron donor, respectively. A steady-state kinetic study demonstrated that the CsPRx proteins followed a saturable, Michaelis-Menten-type equation with the catalytic efficiencies (kcat/Km) ranging from 103 to 104 M-1 s-1, somewhat lower than those for other PRxs studied (104-105 M-1 s-1). The expression patterns and histological distributions specific to CsPRx2 and CsPRx3 might suggest different physiological functions of the antioxidant enzymes in protecting the worms against oxidative damage.
机译：总结我们在中华绒螯蟹中鉴定了2个编码不同2-Cys过氧化物酶（PRxs）的新基因，分别命名为CsPRx2和CsPRx3，该基因侵袭了人类肝胆道。 CsPRx2基因表达随寄生虫的发展而暂时增加，并且在成虫的几乎所有部分（包括皮下和分泌物-分泌物）中都检测到了其蛋白产物。相反，CsPRx3表达暂时保持在基础水平，并很大程度上限制在各种组织/器官的内部。 CsPRx蛋白质的重组形式在硫氧还蛋白或谷胱甘肽（GSH）作为还原当量的情况下表现出对各种氢过氧化物的还原活性，尽管它们分别优选H2O2和GSH作为催化底物和电子供体。稳态动力学研究表明，CsPRx蛋白遵循一个可饱和的Michaelis-Menten型方程，催化效率（kcat / Km）为103至104 M-1 s-1，略低于其他研究的PRx （104-105 M-1 s-1）。 CsPRx2和CsPRx3特有的表达模式和组织学分布可能表明抗氧化酶在保护蠕虫免受氧化损伤方面具有不同的生理功能。

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