...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >Description of the two strains of turkey coccidia Eimeria adenoeides with remarkable morphological variability.
【24h】

Description of the two strains of turkey coccidia Eimeria adenoeides with remarkable morphological variability.

机译：两种具有明显形态变异的火球菌艾美球虫腺苷菌株的描述。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although oocyst morphology was always considered as a reliable parameter for coccidian species discrimination we describe strain variation of turkey coccidia, Eimeria adenoeides, which remarkably exceeds the variation observed in any other Eimeria species. Two strains have been isolated - the first strain maintains the typical oocyst morphology attributed to this species - large and ellipsoidal - while the second strain has small and ovoid oocysts, never described before for this species. Other biological parameters including pathogenicity were found to be similar. Cross-protection between these 2 strains in 2 immunization and challenge experiments was confirmed. Sequencing and analysis of 18S and ITS1 ribosomal DNA revealed a close relationship according to 18S and a relatively distant relationship according to ITS1. Analysis of 18S and ITS1 sequences from commercial turkey coccidiosis vaccines Immucox(R)-T and Coccivac(R)-T revealed that each vaccine contains a different strain of E. adenoeides and that these strains have 18S and ITS1 sequences homologous to the sequences of the strains we have isolated and described. These findings show that diagnostics of turkey coccidia according to oocyst morphology have to be carried out with caution or abolished entirely. Novel PCR-based molecular tools will be necessary for fast and reliable species discrimination.
机译：尽管卵囊形态一直被认为是球虫种鉴别的可靠参数，但我们描述了火球菌，艾美球虫腺苷的菌株变异，该变异明显超过了其他任何艾美球虫物种的变异。已分离出两个菌株-第一个菌株保持了归因于该物种的典型卵囊形态-大且呈椭圆形-而第二个菌株具有小的卵形卵囊，此物种以前从未描述过。发现包括致病性在内的其他生物学参数相似。证实了这2个菌株在2次免疫和攻击实验中的交叉保护。对18S和ITS1核糖体DNA的测序和分析显示，根据18S的关系密切，而根据ITS1的关系较远。对商用火球虫病疫苗Immucox（R）-T和Coccivac（R）-T的18S和ITS1序列进行分析后发现，每种疫苗均含有不同的腺苷菌株，并且这些菌株的18S和ITS1序列与我们已经分离和描述的菌株。这些发现表明，根据卵囊形态对火球菌的诊断必须谨慎进行或完全取消。快速，可靠的物种识别将需要基于PCR的新型分子工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号