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首页> 外文期刊>Parasitology >Sir2-Related Protein 1 from Leishmania amazonensis is a glycosylated NAD+-dependent deacetylase.
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Sir2-Related Protein 1 from Leishmania amazonensis is a glycosylated NAD+-dependent deacetylase.

机译：来自亚马逊利什曼原虫的Sir2相关蛋白1是糖基化的NAD +依赖性脱乙酰基酶。

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摘要

Sirtuin proteins form a family of NAD+-dependent protein deacetylases that are considered potential drug targets against parasites. Here, we present the first characterization of a sirtuin orthologue from Leishmania amazonensis, an aetiological agent of American tegumentary leishmaniasis that has been the subject of many studies focused in the development of therapeutic approaches. The protein has high sequence identity with other Kinetoplastid Silent information regulator 2 Related Protein 1 (Sir2RP1) and was named LaSir2RP1. The gene exists as a single copy, encoding a monomeric protein (LaSir2RP1) of approximately 41 kDa that has NAD+-dependent deacetylase activity. LaSir2RP1 was immunodetected in total protein extracts, in cytoplasmic granules, and in the secreted material of both promastigotes and lesion-derived amastigotes. Analysis of both lectinaffinity purified promastigote and amastigote extracts revealed the presence of a major enriched protein of approximately 66 kDa that was recognized by an anti-LaSir2RP1 serum, suggesting that a parasite sirtuin could be glycosylated in vivo.
机译：Sirtuin蛋白形成NAD +依赖性蛋白脱乙酰基酶家族，被认为是针对寄生虫的潜在药物靶标。在这里，我们介绍了来自美洲利什曼原虫病的病原体亚马逊利什曼原虫的瑟土因直向同源物的第一个特征，该特征已成为许多专注于治疗方法开发的研究的主题。该蛋白与其他Kinetoplastid沉默信息调节剂2相关蛋白1（Sir2RP1）具有高度的序列同一性，被命名为LaSir2RP1。该基因以单拷贝形式存在，编码具有NAD +依赖性脱乙酰酶活性的约41 kDa的单体蛋白（LaSir2RP1）。 LaSir2RP1在总蛋白提取物中，细胞质颗粒中以及在前鞭毛体和病灶衍生的变形虫的分泌物中都被免疫检测到。对电凝亲和力纯化的前鞭毛体和鞭毛体提取物的分析表明，存在约66 kDa的主要富集蛋白，该蛋白被抗LaSir2RP1血清识别，表明寄生虫沉默调节蛋白可能在体内被糖基化。

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