...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >Study of Trypanosoma cruzi epimastigote cell death by NMR-visible mobile lipid analysis
【24h】

Study of Trypanosoma cruzi epimastigote cell death by NMR-visible mobile lipid analysis

机译：核磁共振可见移动脂质分析法研究克鲁氏锥虫副鞭毛细胞死亡

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cell death mechanisms in Trypanosoma cruzi have not been disclosed in detail though different conventional techniques have been used in the classification of parasite-cell death type. Nuclear magnetic resonance (NMR) has successfully been used as a tool to evaluate the onset of apoptosis in a number of higher eukaryote-cell models analysing the ratio of CH2/CH3 integration from the visible mobile lipids (VML). Surprisingly, this versatile non-invasive spectroscopy technique has never been employed with this purpose in T. cruzi. In the present study it is shown that under different parasite death-conditions the ratio CH2/CH3 varied drastically. Thus, T. cruzi epimastigotes in apoptotic conditions increase significantly this ratio while in necrotic as well as in autophagic situations the parasites maintain the VML, CH2/CH3 ratio, in normal values. Additionally, other VML markers commonly used in these studies, such as the change in the region of methyl-choline moiety, -N +(CH3)3, exhibited different particular patterns according to the type of cell death. Our results suggest that the 1H NMR-VML technique is an adequate tool to discriminate different T. cruzi death pathways.
机译：尽管已在寄生虫细胞死亡类型的分类中使用了不同的常规技术，但尚未详细披露克鲁氏锥虫的细胞死亡机制。核磁共振（NMR）已成功地用作评估许多高级真核细胞模型中细胞凋亡的工具，该模型分析了可见移动脂质（VML）中CH2 / CH3整合的比例。出人意料的是，这种通用的非侵入式光谱技术从未在克鲁维酵母中用于此目的。在本研究中表明，在不同的寄生虫死亡条件下，CH2 / CH3的比值急剧变化。因此，在凋亡条件下的克鲁氏疟原虫淫羊s显着增加了该比率，而在坏死以及自噬情况下，寄生虫将VML，CH2 / CH3比率维持在正常值。此外，这些研究中常用的其他VML标记，例如甲基胆碱部分-N +（CH3）3的区域变化，根据细胞死亡的类型表现出不同的特定模式。我们的结果表明1H NMR-VML技术是区分不同克鲁斯锥虫死亡途径的适当工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号