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首页> 外文期刊>Biomaterials >Interactions between resin monomers and commercial composite resins with human saliva derived esterases.
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Interactions between resin monomers and commercial composite resins with human saliva derived esterases.

机译：树脂单体和市售复合树脂与人唾液衍生酯酶之间的相互作用。

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摘要

Cholesterol esterase (CE) and pseudocholinesterase (PCE) have been reported to degrade commercial and model composite resins containing bisphenylglycidyl dimethacrylate (BisGMA), triethylene glycol dimethacrylate (TEGDMA) or the latter in combination with urethane modified BisGMA monomer systems. In addition, human saliva has been shown to contain esterase like activities similar to CE and PCE. Hence, it was the aim of the current study to determine to what extent human saliva could degrade two common commercial composite resins (Z250 from 3M Inc. and Spectrum TPH from L.D. Caulk) which contain the above monomer systems. Saliva samples from different volunteers were collected, processed, pooled, and freeze-dried. TEGDMA and BisGMA monomers were incubated with human saliva derived esterase activity (HSDEA) and their respective hydrolysis was monitored using high performance liquid chromatography (HPLC). Both monomers were completely hydrolyzed within 25 h by HSDEA. Photopolymerized composites were incubated with buffer or human saliva (pH 7.0 and 37 C) for 2, 8 and 16 days. The incubation solutions were analyzed using HPLC and mass spectrometry. Surface morphology characterization was carried out using scanning electron microscopy. Upon biodegradation, the Z250 composite yielded higher amounts of BisGMA and TEGDMA related products relative to the TPH composite. However, there were higher amounts of ethoxylated bis-phenol A released from the TPH material. In terms of total mass of products released, human saliva demonstrated a greater ability to degrade Z250. In summary, HSDEA has been shown to contain esterase activities that can readily catalyze the biodegradation of current commercial composite resins.
机译：据报道，胆固醇酯酶（CE）和伪胆碱酯酶（PCE）可以降解含有双苯基缩水甘油基二甲基丙烯酸酯（BisGMA），三乙二醇二甲基丙烯酸酯（TEGDMA）或后者与氨基甲酸酯改性的BisGMA单体系统组合的商业和模型复合树脂。另外，已经证明人类唾液含有类似于CE和PCE的酯酶样活性。因此，本研究的目的是确定人的唾液在多大程度上可以降解包含上述单体体系的两种常见的商业复合树脂（3M Inc.的Z250和L.D. Caulk的Spectrum TPH）。收集，处理，合并和冷冻干燥来自不同志愿者的唾液样品。将TEGDMA和BisGMA单体与人唾液衍生的酯酶活性（HSDEA）进行孵育，并使用高效液相色谱（HPLC）监控它们各自的水解。两种单体在25小时内被HSDEA完全水解。将光聚合的复合物与缓冲液或人唾液（pH 7.0和37 C）孵育2、8和16天。使用HPLC和质谱分析孵育溶液。使用扫描电子显微镜进行表面形态表征。生物降解后，相对于TPH复合材料，Z250复合材料产生的BisGMA和TEGDMA相关产品的含量更高。但是，从TPH材料中释放出更多的乙氧基化双酚A。就释放的产品总量而言，人类唾液具有更高的降解Z250的能力。总而言之，HSDEA已显示出具有酯酶活性，可轻易催化目前市售复合树脂的生物降解。

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