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首页> 外文期刊>Biomaterials >Surface passivation of a microfluidic device to glial cell adhesion: a comparison of hydrophobic and hydrophilic SAM coatings.
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Surface passivation of a microfluidic device to glial cell adhesion: a comparison of hydrophobic and hydrophilic SAM coatings.

机译：微流体装置对神经胶质细胞粘附的表面钝化：疏水性和亲水性SAM涂层的比较。

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Cell adhesion in a microfluidic structure can lead to catastrophic flow problems due to the comparable size of the cell with the microfabricated device. Such issues are important in the growing research area involving the merging of biological materials and MEMS devices. We have examined the surface compatibility of uncoated and coated microfabricated glass and semiconductor surfaces under static solution (cell culture) and flow experiments (microfluidic device) using glial (astrocyte and glioblastoma) cells. Bare semiconductor and glass surfaces were most attractive to cell adhesion, promoting biofouling under both static and flow conditions. Passivation of the surfaces was performed with silane coupling agents octadecyltrimethoxysilane (OTMS) or N-(triethoxysilylpropyl)-O-polyethylene oxide urethane (TESP) on SiO2 surfaces via self-assembled monolayer (SAM) deposition. The hydrophilic TESP coating was effective at inhibiting biofouling of the microfluidic structure, allowing greater than several minutes of fluid flow. The hydrophobic OTMS coating, on the other hand, promoted cell adhesion leading to restricted flow within a few minutes. Interestingly, under cell culture conditions the TESP surface exhibited biocompatible properties for glial cell adhesion and proliferation, in contrast to the OTMS surface which resisted cell growth. These studies suggest that cell adhesion is dependent upon the time domain of the cell-surface interaction.
机译：由于与微制造装置相当的细胞尺寸，微流体结构中的细胞粘附可导致灾难性的流动问题。在涉及生物材料和MEMS器件融合的不断发展的研究领域中，此类问题非常重要。我们已经在使用胶质细胞（星形胶质细胞和胶质母细胞瘤）的静态溶液（细胞培养）和流动实验（微流体装置）下检查了未镀膜和镀膜的超细玻璃和半导体表面的表面相容性。裸露的半导体和玻璃表面最吸引细胞粘附，在静态和流动条件下都促进了生物积垢。使用硅烷偶联剂十八烷基三甲氧基硅烷（OTMS）或N-（三乙氧基甲硅烷基丙基）-O-聚环氧乙烷氨基甲酸酯（TESP）通过自组装单层（SAM）沉积对表面进行钝化。亲水性TESP涂层可有效抑制微流体结构的生物结垢，使流体流过几分钟。另一方面，疏水性OTMS涂层促进了细胞粘附，导致几分钟内流量受限。有趣的是，与可抵抗细胞生长的OTMS表面相反，TESP表面在胶质细胞粘附和增殖方面表现出生物相容性。这些研究表明，细胞粘附取决于细胞表面相互作用的时域。

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