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首页> 外文会议>Conference on Biomedical Instrumentation Based on Micro- and Nanotechnology Jan 24-25, 2001, San Jose, USA >Glial cell adhesion and protein adsorption on SAM coated semiconductor and glass surfaces of a microfluidic structure
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Glial cell adhesion and protein adsorption on SAM coated semiconductor and glass surfaces of a microfluidic structure

机译：胶质细胞粘附和蛋白质吸附在SAM涂层的半导体和微流体结构的玻璃表面上

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The development of microsystems that merge biological materials with microfabricated structures is highly dependent on the successful interfacial interactions between these innately incompatible materials. Surface passivation of semiconductor and glass surfaces with thin organic films can attenuate the adhesion of proteins and cells that lead to biofilm formation and biotbuling of fluidic structures. We have examined the adhesion of glial cells and serum albumin proteins to microfabricated glass and semiconductor surfaces coated with self-assembled monolayers (SAM) of octadecyltrimethoxysilane (OTMS) and N-(triethoxysilylpropyl)-O-polyethylene oxide urethane (TESP), to evaluate the biocompatibility and surface passivation those coatings provide. These films were exposed to solutions containing serum albumin proteins (4 mg/mL), glial cells in culturing media, and glial cells under fluid flow. While the OTMS surface resisted cell spreading and growth under culture conditions, the same surface induced biofouling in a cell flow experiment with a microfluidic structure. Interestingly, the TESP surface, which was supportive of cell adhesion and proliferation under cell culturing conditions, effectively passivated the microfluidic structure to cell adhesion and biofouling. The results suggest that the cell adhesion process is not only dependent on the chemistry of the surface but also on the time allotted to the cell to probe the surface.
机译：将生物材料与微细结构融合的微系统的开发高度依赖于这些天生不相容的材料之间的成功界面相互作用。带有有机薄膜的半导体和玻璃表面的表面钝化可以减弱蛋白质和细胞的粘附力，从而导致生物膜的形成和流体结构的生物絮凝。我们已经检查了神经胶质细胞和血清白蛋白对微型玻璃和半导体表面的粘附性，以评估十八烷基三甲氧基硅烷（OTMS）和N-（三乙氧基甲硅烷基丙基）-O-聚环氧乙烷聚氨酯（TESP）的自组装单层（SAM）涂层这些涂层提供的生物相容性和表面钝化作用。将这些膜暴露于含有血清白蛋白（4 mg / mL），培养基中的神经胶质细胞和流体流动下的神经胶质细胞的溶液中。尽管OTMS表面在培养条件下可阻止细胞扩散和生长，但同一表面在具有微流体结构的细胞流动实验中诱导了生物积垢。有趣的是，在细胞培养条件下支持细胞粘附和增殖的TESP表面有效地使微流体结构钝化为细胞粘附和生物污染。结果表明，细胞粘附过程不仅取决于表面的化学性质，还取决于分配给细胞探测表面的时间。

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