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首页> 外文期刊>Biomaterials >The influence of plasma proteins and platelets on oxygen radical production and F-actin distribution in neutrophils adhering to polymer surfaces.
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The influence of plasma proteins and platelets on oxygen radical production and F-actin distribution in neutrophils adhering to polymer surfaces.

机译：血浆蛋白和血小板对粘附于聚合物表面的嗜中性粒细胞中氧自由基产生和F-肌动蛋白分布的影响。

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摘要

It is well known that blood cell interactions with artificial surfaces might have deleterious effects on host tissue, however, the mechanisms involved are far from understood. In this study, neutrophil-platelet interaction on uncoated or protein-coated polymer surfaces was investigated. Cell spreading, reorganization of actin filaments and release of oxygen metabolites (measured as luminol-amplified chemiluminescence) were used as criteria for cell activation on positively charged, hydrophilic 1,2-diaminocyclohexane, and negatively charged, hydrophobic hexamethylene-disiloxane. The model surfaces were made by radio frequency plasma discharge polymerization. Neutrophil contact with the uncoated polymers induced a prolonged generation of oxygen radicals. Precoating of the polymer surfaces with human serum albumin (HSA) or fibrinogen, markedly reduced neutrophil activation, whereas coating with human immunoglobulin G (IgG), a well-known opsonin, resulted in significantly higher levels of cell activation. Consequently, protein coating overruled the activating effects of the polymer surfaces. The presence of unstimulated or thrombin-stimulated platelets markedly increased the reactivity of neutrophils against fibrinogen- and IgG-coated surfaces. However, neutrophils remained relatively unreactive in the presence of platelets on HSA-treated surfaces. Comparison of the different types of surfaces used, reveals a correlation between the degree of cell spreading, reorganization of the actin cytoskeleton and the amount of oxygen radicals produced. Our results suggest that the acute inflammatory reaction on a biomaterial surface is highly dependent on the nature and composition of the first adsorbed protein layer and the extent of platelet activation.
机译：众所周知，血细胞与人造表面的相互作用可能对宿主组织产生有害影响，但是，所涉及的机制尚不清楚。在这项研究中，研究了未包被或蛋白包被的聚合物表面上的嗜中性粒细胞-血小板相互作用。细胞扩散，肌动蛋白丝重组和氧代谢产物的释放（测量为鲁米诺增强化学发光）被用作在带正电的亲水性1,2-二氨基环己烷和带负电的疏水性六亚甲基-二硅氧烷上进行细胞活化的标准。通过射频等离子体放电聚合制备模型表面。中性粒细胞与未涂覆的聚合物接触导致氧自由基的延长生成。用人血清白蛋白（HSA）或纤维蛋白原对聚合物表面进行预涂层可显着降低中性粒细胞的活化，而用人免疫球蛋白G（IgG）（一种众所周知的调理素）进行涂层则可显着提高细胞活化水平。因此，蛋白质涂层否决了聚合物表面的活化作用。未刺激的或凝血酶刺激的血小板的存在显着增加了中性粒细胞对纤维蛋白原和IgG包被表面的反应性。然而，在HSA处理的表面上存在血小板的情况下，中性粒细胞仍然相对不反应。比较所使用的不同类型的表面，揭示了细胞扩散程度，肌动蛋白细胞骨架的重组与产生的氧自由基之间的相关性。我们的结果表明，生物材料表面的急性炎症反应高度依赖于第一吸附蛋白层的性质和组成以及血小板活化的程度。

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