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Investigations on mass production of the potato rot nematode, Ditylenchus destructor (Thorne, 1945) using different monoxenic cultures

机译:使用不同一氧化碳培养物大规模生产马铃薯腐烂线虫Ditylenchus破坏物的研究(Thorne,1945年)

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摘要

The reproduction of an Iranian population of Ditylenchus destructor Thorne, 1945 was investigated in different media under laboratory conditions to identify the best medium and fungal host for mass production of this nematode. Four media, including three handmade PDA media (Potato Dextrose Agar), prepared from extracts of three cultivars of potato (Santana, Ramos & Maradona) and one medium of OMA (Oat Meal Agar), were used as substrates to grow fungi isolated from potato fields, including different anastomozis group of Rhizoctonia solani (AG3, AG4, AG5, AG6, AGD, AG9), Phytophthora capsici, Botiytis cinerea and Fusarium oxysporum f.sp. lycopersici. These fungal cultures were then inoculated with the nematodes and maintained at 20 and 25 deg C. The results showed that the nematode fed and reproduced more on R. solani (AG3, AG4 and AGD) and F. oxysporum f.sp. lycopersici than on other fungi. However, R. solani (AG3 and AG5) and P. capsici at 25 deg C were also suitable for mass production of this nematode. The reproduction of the nematode was greater at 25 deg C than at 20 deg C.
机译:在实验室条件下,在不同介质中对1945年Ditylenchus破坏者索恩的伊朗种群的繁殖进行了研究,以确定该线虫大规模生产的最佳培养基和真菌宿主。四种培养基,包括三种手工PDA培养基(马铃薯葡萄糖琼脂),是从三种马铃薯(桑塔纳,拉莫斯和马拉多纳)的提取物中制备的,而另一种是OMA(燕麦粉琼脂),是从马铃薯中分离出真菌的基质。田地,包括茄根霉不同的吻合组(AG3,AG4,AG5,AG6,AGD,AG9),辣椒疫霉,灰霉菌和尖孢镰刀菌(Fusarium oxysporum f.sp.)。 lycopersici。然后将这些真菌培养物用线虫接种,并保持在20和25℃。结果表明,线虫在solani R.solani(AG3,AG4和AGD)和F. oxysporum f.sp.上进食并繁殖更多。 lycopersici比在其他真菌上。然而,在25℃下的sol。R. solani(AG3和AG5)和辣椒辣椒也适用于该线虫的批量生产。线虫的繁殖在25摄氏度比在20摄氏度更大。

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