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首页> 外文期刊>Pediatrics: Official Publication of the American Academy of Pediatrics >Translational research in pediatrics III: Bronchoalveolar lavage
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Translational research in pediatrics III: Bronchoalveolar lavage

机译：儿科转化研究III：支气管肺泡灌洗

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摘要

The role of flexible bronchoscopy and bronchoalveolar lavage (BAL) for the care of children with airway and pulmonary diseases is well established, with collected BAL fluid most often used clinically for microbiologic pathogen identification and cellular analyses. More recently, powerful analytic research methods have been used to investigate BAL samples to better understand the pathophysiological basis of pediatric respiratory disease. Investigations have focused on the cellular components contained in BAL fluid, such as macrophages, lymphocytes, neutrophils, eosinophils, and mast cells, as well as the noncellular components such as serum molecules, inflammatory proteins, and surfactant. Molecular techniques are frequently used to investigate BAL fluid for the presence of infectious pathologies and for cellular gene expression. Recent advances in proteomics allow identification of multiple protein expression patterns linked to specific respiratory diseases, whereas newer analytic techniques allow for investigations on surfactant quantification and function. These translational research studies on BAL fluid have aided our understanding of pulmonary inflammation and the injury/repair responses in children. We review the ethics and practices for the execution of BAL in children for translational research purposes, with an emphasis on the optimal handling and processing of BAL samples.
机译：灵活的支气管镜检查和支气管肺泡灌洗（BAL）在照顾气道和肺部疾病的儿童中的作用已得到充分确立，收集的BAL液最常用于临床上的微生物病原体鉴定和细胞分析。最近，强大的分析研究方法已用于调查BAL样本，以更好地了解小儿呼吸系统疾病的病理生理基础。研究集中于BAL液中所含的细胞成分，例如巨噬细胞，淋巴细胞，嗜中性粒细胞，嗜酸性粒细胞和肥大细胞，以及非细胞成分，例如血清分子，炎性蛋白和表面活性剂。分子技术经常用于研究BAL液是否存在感染性病理和细胞基因表达。蛋白质组学的最新进展允许鉴定与特定呼吸系统疾病有关的多种蛋白质表达模式，而较新的分析技术允许对表面活性剂的定量和功能进行研究。这些对BAL液的转化研究有助于我们了解儿童的肺部炎症和损伤/修复反应。我们审查了儿童进行BAL的伦理学和实践，以进行转化研究，重点是BAL样品的最佳处理和加工。

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