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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Association between Expression Levels of CA 19-9 and N-ACETYLGLUCOSAMINE-BETA;1,3-GALACTOSYLTRANSFERASE 5 Gene in Human Pancreatic Cancer Tissue.
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Association between Expression Levels of CA 19-9 and N-ACETYLGLUCOSAMINE-BETA;1,3-GALACTOSYLTRANSFERASE 5 Gene in Human Pancreatic Cancer Tissue.

机译：人胰腺癌组织中CA 19-9和N-ACETYLGLUCOSAMINE-BETA; 1,3-GALACTOSYLTRANSFERASE 5基因表达水平的关联。

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OBJECTIVE: CA 19-9, equivalent to Sialyl Lewis(a) antigen, is a well-known tumor marker in pancreatic cancer. At the initial step of the biosynthesis of CA 19-9, N-acetylglucosamine-beta1,3-galactosyltransferase (beta3Gal-T) transfers galactose to N-acetylglucosamine (GlcNAc). Recently, beta3Gal-T5 has been presumed to be related to the formation of the type 1 chain in an in vitro experiment in terms of kinetic enzyme characterization. The purpose of this study was to investigate which beta3Gal-T is related to the synthesis of CA 19-9 in human pancreatic cancer tissues. METHODS: We examined BETA;3GAL-T1, T2, T3, T4, and BETA;3GAL-T5 mRNA expressions in 13 noncancerous and cancerous tissues of the human pancreas using real-time polymerase chain reaction, and compared those gene expression levels with the immunoreactivity of CA 19-9 and its precursor DUPAN-2 in cancerous tissues. RESULTS:BETA;3GAL-T5 gene expression significantly augmented in cancerous tissues, when compared with the adjacent noncancerous tissues. Additionally, there was a good correlation between BETA;3GAL-T5 gene transcription levels and immunohistochemical grades of CA 19-9 or its precursor DUPAN-2 in cancerous tissues. However, no correlation was observed between BETA;3GAL-T1, T2, T3, and BETA;3GAL-T4 gene expression levels and CA 19-9 or DUPAN-2 immunoreactive grades in cancerous tissue. CONCLUSION: beta3Gal-T5 is presumed to be responsible for the synthesis of CA 19-9 in pancreatic cancer tissue.
机译：目的：CA 19-9相当于唾液酸化Lewis（a）抗原，是胰腺癌中众所周知的肿瘤标志物。在CA 19-9生物合成的初始步骤，N-乙酰氨基葡萄糖-β1,3-半乳糖基转移酶（beta3Gal-T）将半乳糖转移至N-乙酰氨基葡萄糖（GlcNAc）。最近，就体外酶实验而言，据推测β3Gal-T5与1型链的形成有关。这项研究的目的是调查哪种beta3Gal-T与人类胰腺癌组织中CA 19-9的合成有关。方法：我们使用实时聚合酶链反应检测了人类胰腺13种非癌和癌组织中的BETA; 3GAL-T1，T2，T3，T4和BETA; 3GAL-T5 mRNA表达，并将这些基因表达水平与癌组织中CA 19-9及其前体DUPAN-2的免疫反应性。结果：与邻近的非癌组织相比，BETA; 3GAL-T5基因表达在癌组织中显着增加。另外，癌组织中BETA; 3GAL-T5基因的转录水平与CA 19-9或其前体DUPAN-2的免疫组化等级之间存在良好的相关性。但是，在癌组织中，BETA; 3GAL-T1，T2，T3和BETA; 3GAL-T4基因表达水平与CA 19-9或DUPAN-2免疫反应等级之间没有相关性。结论：β3Gal-T5被认为是胰腺癌组织中CA 19-9合成的原因。

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