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首页> 外文期刊>Biomaterials >The linker-free covalent attachment of collagen to plasma immersion ion implantation treated polytetrafluoroethylene and subsequent cell-binding activity.
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The linker-free covalent attachment of collagen to plasma immersion ion implantation treated polytetrafluoroethylene and subsequent cell-binding activity.

机译：胶原蛋白与血浆浸没离子注入处理后的聚四氟乙烯的无连接子共价连接以及随后的细胞结合活性。

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摘要

It is desirable that polymers used for the fabrication of prosthetic implants promote biological functions such as cellular adhesion, differentiation and viability. In this study, we have used plasma immersion ion implantation (PIII) to modify the surface of polytetrafluoroethylene (PTFE), thereby modulating the binding mechanism of collagen. The amount of collagen bound to the polymer surface following PIII-treatment was similar to that bound by non-covalent physisorption. In a manner consistent with previous enzyme and tropoelastin binding data, the collagen bound to the PIII-treated PTFE surface was resistant to sodium dodecyl sulfate (SDS) elution whilst collagen bound to the untreated surface was fully removed. This demonstrates the capability of PIII-treated surfaces to covalently attach collagen without employing chemical linking molecules. Only the collagen bound to the PIII-treated PTFE surface supported human dermal fibroblast attachment and spreading. This indicates that collagen on the PIII-treated surface possesses increased adhesive activity as compared to that on the untreated surface. Cell adhesion was inhibited by EDTA when the collagen was bound to PIII-treated PTFE, as expected for integrin involvement. Additionally this adhesion was sensitive to the conformation of the bound collagen. Increased actin cytoskeletal assembly was observed on cells spreading onto collagen-coated PIII-treated PTFE compared to the collagen-coated untreated PTFE. These data demonstrate the retention of collagen's biological properties following its attachment to PIII-treated PTFE, suggesting advantages for tissue engineering and prosthetic design.
机译：理想的是，用于制造假体的聚合物促进生物功能，例如细胞粘附，分化和存活。在这项研究中，我们已经使用等离子体浸没离子注入（PIII）来修饰聚四氟乙烯（PTFE）的表面，从而调节胶原蛋白的结合机制。 PIII处理后，与聚合物表面结合的胶原蛋白的量与非共价物理吸附结合的胶原蛋白的量相似。以与先前的酶和原弹性蛋白结合数据一致的方式，结合至PIII处理的PTFE表面的胶原蛋白对十二烷基硫酸钠（SDS）洗脱具有抵抗力，而结合至未处理表面的胶原蛋白则被完全去除。这证明了经PIII处理的表面无需化学连接分子即可共价附着胶原的能力。只有结合到PIII处理的PTFE表面的胶原蛋白才支持人类皮肤成纤维细胞的附着和扩散。这表明与未经处理的表面相比，经PIII处理的表面上的胶原蛋白具有增强的粘合活性。当胶原蛋白结合到PIII处理的PTFE上时，EDTA会抑制细胞粘附，这是整合素的作用所期望的。另外，这种粘附对结合的胶原的构象敏感。与涂有胶原涂层的未经处理的PTFE相比，在铺展在涂有胶原涂层的PIII处理的PTFE上的细胞上观察到肌动蛋白细胞骨架装配的增加。这些数据表明，将胶原蛋白附着到经过PIII处理的PTFE之后，胶原蛋白的生物学特性得以保留，这提示了组织工程和假体设计的优势。

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