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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Laser capture microdissection: methodical aspects and applications with emphasis on immuno-laser capture microdissection.
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Laser capture microdissection: methodical aspects and applications with emphasis on immuno-laser capture microdissection.

机译：激光捕获显微切割：方法方面和应用，重点是免疫激光捕获显微切割。

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摘要

Laser capture microdissection (LCM) is an easy, extremely fast and versatile method for the isolation of morphologically defined cell populations from complex primary tissues for molecular analyses. However, the optical resolution is limited due to the use of dried sections without coverslip necessary for tissue capture, and routine stains such as hematoxylin and eosin are sometimes insufficient for precise microdissection, especially in tissues with diffuse intermingling of different cell types and lack of easily discernible architectural features. Therefore, several groups have adapted immunohistochemical staining techniques for LCM. In addition to providing high contrast targets for microdissection, immunostaining allows selection of cells not only according to morphological, but also phenotypical and functional criteria. In order to allow reliable tissue transfer on one hand and preserve the integrity of the target of analysis such as DNA, RNA and proteins on the other hand, immunostaining protocols have to be modified for the purposes of LCM. The following review gives an overview of immuno-LCM and describes some applications, e.g. in the field of hematopathology. Copyright 2001 S. Karger AG, Basel
机译：激光捕获显微切割术（LCM）是一种从复杂的原始组织中分离出形态定义的细胞群体以进行分子分析的简便，快速且通用的方法。但是，光学分辨率受到限制，这是因为使用了干燥的切片，而没有组织捕获所需的盖玻片，并且常规染色剂（如苏木精和曙红）有时不足以进行精确的显微切割，尤其是在具有不同细胞类型的弥散性混合且缺乏​​容易性的组织中明显的建筑特征。因此，有几组针对LCM采用了免疫组织化学染色技术。除了为显微解剖提供高对比度目标外，免疫染色不仅可以根据形态学，还可以根据表型和功能标准选择细胞。为了一方面允许可靠的组织转移并另一方面保持分析目标（如DNA，RNA和蛋白质）的完整性，出于LCM的目的，必须修改免疫染色方案。以下评论概述了免疫LCM，并描述了一些应用，例如在血液病理学领域。版权所有2001 S. Karger AG，巴塞尔

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