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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Recovering DNA and optimizing PCR conditions from microdissected formalin-fixed and paraffin-embedded materials.
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Recovering DNA and optimizing PCR conditions from microdissected formalin-fixed and paraffin-embedded materials.

机译：从显微解剖的福尔马林固定石蜡包埋材料中回收DNA并优化PCR条件。

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摘要

Microdissection is a powerful technique in molecular pathology and genetic investigations. To detect genetic alterations such as gene mutation or deletion from tumor specimen, the purity of target cells is extremely critical. Unwanted cell contamination will dramatically dilute the detectable level of the abnormality. The major obstacle in clinical research is to obtain sufficient and qualified DNA from a small amount of formalin-fixed and paraffin-embedded materials. We have successfully modified our previous protocols and overcome the difficulties of recovery of DNA. After these modifications, almost every single formalin-fixed and paraffin-embedded specimen has been successfully amplified in the required DNA region. Copyright 2001 S. Karger AG, Basel
机译：显微解剖是分子病理学和遗传学研究中的一项强大技术。为了检测遗传改变，例如基因突变或肿瘤标本的缺失，靶细胞的纯度至关重要。有害的细胞污染将大大稀释异常的可检测水平。临床研究的主要障碍是从少量福尔马林固定和石蜡包埋的材料中获得足够的合格DNA。我们已经成功修改了以前的方案，并克服了DNA回收的困难。经过这些修饰后，几乎每一个福尔马林固定和石蜡包埋的标本都已在所需的DNA区域成功扩增。版权所有2001 S. Karger AG，巴塞尔

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