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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Diagnosis of papillary thyroid carcinoma is facilitated by using an RT-PCR approach on laser-microdissected archival material to detect RET oncogene activation.
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Diagnosis of papillary thyroid carcinoma is facilitated by using an RT-PCR approach on laser-microdissected archival material to detect RET oncogene activation.

机译：通过在激光显微切割的存档材料上使用RT-PCR方法来检测RET癌基因的活化，可促进乳头状甲状腺癌的诊断。

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OBJECTIVE: The purpose of this study was to investigate the value of the expression of the RET oncogene (rearranged during transfection) in papillary thyroid carcinomas (PTC) and its variants in the differential diagnosis of thyroid neoplasias. According to the literature RET oncogene activation by chromosomal rearrangements has been exclusively implicated in PTCs. METHODS: To establish the incidence of RET activation in PTCs we used 5- to 10-microm sections from archival paraffin blocks. Either parts of the tissue slices were manually dissected or a few distinct cells were microdissected by laser-mediated manipulation with the Robot-MicroBeam system. RNA was extracted from paraffin-embedded thyroid tumors and the corresponding normal tissue. RT and nested PCR were performed using primers for RET/PTC1, PTC2 and PTC3, or for RET exons 12 and 13. PCR products were resolved by gel electrophoresis. RESULTS: We detected RET transcription in approximately 85% of the PTCs including follicular variants and in isolated cells of the same tissues, but not in nonmalignant thyroid tissue. CONCLUSIONS: Our method may serve as an additional diagnostic tool to characterize ambiguous neoplasias and to identify especially nonpapillary, i.e. follicular tumors, as papillary carcinomas. Additionally, this study has demonstrated that expressed genes can be analyzed from routine histopathological tissue slides or pooled single cells. Large retrospective studies can also be performed with this method. Copyright 2001 S. Karger AG, Basel
机译：目的：研究RET癌基因（转染过程中重新排列）在乳头状甲状腺癌（PTC）及其变种中的表达在甲状腺肿瘤的鉴别诊断中的价值。根据文献，通过染色体重排的RET致癌基因激活仅与PTC相关。方法：为了确定PTC中RET活化的发生率，我们使用了档案石蜡块的5至10微米切片。用Robot-MicroBeam系统通过激光介导的操作，手动解剖组织切片的任一部分或显微切割一些不同的细胞。从石蜡包埋的甲状腺肿瘤和相应的正常组织中提取RNA。使用RET / PTC1，PTC2和PTC3或RET外显子12和13的引物进行RT和巢式PCR。PCR产物通过凝胶电泳分离。结果：我们检测到大约5％的PTC（包括卵泡变体）和相同组织的分离细胞中检测到RET转录，但在非恶性甲状腺组织中未检测到。结论：我们的方法可以作为诊断模棱两可的肿瘤的特征的附加诊断工具，特别是将非乳头状即滤泡状肿瘤鉴别为乳头状癌。此外，这项研究表明，可以从常规组织病理学切片或合并的单细胞中分析表达的基因。大型回顾性研究也可以用这种方法进行。版权所有2001 S. Karger AG，巴塞尔

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