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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >CD50 and CD62L adhesion receptor expression on naive (CD45RA+) and memory (CD45RO+) T lymphocytes in the elderly.
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CD50 and CD62L adhesion receptor expression on naive (CD45RA+) and memory (CD45RO+) T lymphocytes in the elderly.

机译：老年人中幼稚（CD45RA +）和记忆（CD45RO +）T淋巴细胞的CD50和CD62L粘附受体表达。

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摘要

A complex reshaping characterizes cellular immunity in the elderly. In particular, the hallmark of the "senescence" of the T cell compartment is a decrease in the proportion of CD45RA+ naive T lymphocytes concomitantly with an expansion of CD45RO+ memory T cells. However, in addition to age-dependent changes in their representation, phenotypical and functional anomalies also characterize naive and memory T cell populations in the elderly. Since cell adhesion molecules (CAMs) are multifunctional receptors which play important roles not only in cell-to-cell and cell-to-matrix interactions but also in signal transduction and cell activation, we analysed, by means of a three-colour flow cytometry method, the proportion, absolute number and density expression or mean fluorescence intensity (MFI) of CD50 (ICAM-3) and CD62L (L-selectin homing receptor) adhesion receptors on CD45RA+ and CD45RO+ peripheral blood CD3+ T cell subsets from 10 healthy elderly subjects and 10 young controls. Our aim was to investigate age-dependent changes in the expression pattern of these CAMs on naive and memory lymphocytes which might contribute to the remodelling of the immune system in the elderly. We considered the mean values +/- standard deviations of the percentage, absolute number and MFI of positive cells. The percentage of naive T cells expressing CD50 was not significantly modified in aged (94.8 +/- 5.0%) compared to young individuals (97.8 +/- 3.2%). On the contrary, the percentage of memory T cells exhibiting CD50 was lower in elderly than young donors (92.0 +/- 6.4 vs. 98.3 +/- 2.2%; p < 0.01). The percentage of naive T cells expressing CD62L was decreased in the elderly donors (53.3 +/- 18.8 vs. 80.8 +/- 11.0%; p < 0.001), whereas the proportion of CD62L+ memory T lymphocytes was substantially comparable between the two age groups (63.5 +/- 15.7 vs. 54.7 +/- 12.3%). The absolute number per mm(3) of CD50+ naive T cells from aged individuals was decreased (251.9 +/- 141.9 vs. 621.8 +/- 238.0/mm(3); p < 0.001), whereas memory peripheral blood T lymphocytes expressing CD50 were substantially unchanged (863.8 +/- 260.9 vs. 802.7 +/- 139.6/mm(3)). On the contrary, the absolute numbers per mm(3) of naive and memory peripheral blood T lymphocytes exhibiting CD62L were respectively decreased (190.8 +/- 133.4/mm(3)) and increased (515.1 +/- 146.8/mm(3)) in elderly donors compared to young controls (601.3 +/- 129.1 and 351.8 +/- 195.0/mm(3); p < 0.001 and p < 0.05, respectively). Finally, CD50 MFI values of naive as well as memory T cell subpopulations from aged subjects were increased compared to young donors (14.0 +/- 2.0 vs. 9.8 +/- 1.2 and 14.0 +/- 2.0 vs. 11.6 +/- 1.3; p < 0.001 and p < 0.01, respectively). CD62L was also overexpressed in both naive (8.4 +/- 1.6 vs. 6.7 +/- 1.4; p < 0.05) and memory (10.3 +/- 2.5 vs. 5.4 +/- 1.1; p < 0.001) T subsets in the elderly. CD50 and CD62L upregulation could be interpreted as a compensatory mechanism for a decreased responsiveness and a greater requirement for activation signals rather than an age-related anomaly. Copyright 2001 S. Karger AG, Basel
机译：复杂的重塑是老年人细胞免疫的特征。特别是，T细胞区室“衰老”的标志是CD45RA +幼稚T淋巴细胞比例的减少与CD45RO +记忆T细胞的扩增同时发生。然而，除了代表年龄的变化外，表型和功能异常也表征了老年人的幼稚和记忆T细胞群。由于细胞粘附分子（CAMs）是多功能受体，不仅在细胞间和细胞间相互作用中起着重要作用，而且在信号转导和细胞激活中也起着重要的作用，因此我们通过三色流式细胞仪进行了分析。方法，来自10位健康老年受试者的CD45RA +和CD45RO +外周血CD3 + T细胞亚群上CD50（ICAM-3）和CD62L（L-选择素归巢受体）粘附受体的比例，绝对数量，密度表达或平均荧光强度（MFI）和10个年轻控件。我们的目的是研究天真和记忆淋巴细胞上这些CAM的表达模式的年龄依赖性变化，这可能有助于老年人免疫系统的重塑。我们考虑了阳性细胞的百分比，绝对数和MFI的平均值+/-标准偏差。与年轻个体（97.8 +/- 3.2％）相比，年龄（94.8 +/- 5.0％）的未表达CD50的幼稚T细胞的百分比没有明显改变。相反，老年人中表现出CD50的记忆T细胞百分比低于年轻供者（92.0 +/- 6.4对98.3 +/- 2.2％; p <0.01）。在老年供体中，表达CD62L的幼稚T细胞的百分比降低了（53.3 +/- 18.8 vs. 80.8 +/- 11.0％; p <0.001），而在两个年龄组中，CD62L +记忆T淋巴细胞的比例基本相当（63.5 +/- 15.7与54.7 +/- 12.3％）。老年个体的CD50 +幼稚T细胞的每mm（3）绝对数量减少了（251.9 +/- 141.9与621.8 +/- 238.0 / mm（3）; p <0.001），而表达CD50的记忆外周血T淋巴细胞基本上没有变化（863.8 +/- 260.9 vs.802.7 +/- 139.6 / mm（3））。相反，显示出CD62L的幼稚和记忆外周血T淋巴细胞的每mm（3）绝对数分别降低（190.8 +/- 133.4 / mm（3））和增加（515.1 +/- 146.8 / mm（3）） ）与年轻对照组相比（601.3 +/- 129.1和351.8 +/- 195.0 / mm（3）; p分别为<0.001和p <0.05）。最后，与年轻的供体相比，幼稚的幼稚和记忆T细胞亚群的CD50 MFI值增加了（14.0 +/- 2.0 vs. 9.8 +/- 1.2和14.0 +/- 2.0 vs. 11.6 +/- 1.3; p <0.001和p <0.01分别）。 CD62L在天真的T亚型（8.4 +/- 1.6 vs. 6.7 +/- 1.4; p <0.05）和记忆力（10.3 +/- 2.5 vs. 5.4 +/- 1.1; p <0.001）中也都过表达。 CD50和CD62L的上调可以解释为一种补偿机制，用于降低反应性和对激活信号的更高要求，而不是与年龄有关的异常。版权所有2001 S. Karger AG，巴塞尔

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