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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Influence of insulin and muscle fiber type in nepsilon-(carboxymethyl)-lysine accumulation in soleus muscle of rats with streptozotocin-induced diabetes mellitus.
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Influence of insulin and muscle fiber type in nepsilon-(carboxymethyl)-lysine accumulation in soleus muscle of rats with streptozotocin-induced diabetes mellitus.

机译：胰岛素和肌纤维类型对链脲佐菌素诱发的糖尿病大鼠比目鱼肌中nepsilon-（羧甲基）-赖氨酸蓄积的影响。

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BACKGROUND: Nepsilon-(carboxymethyl)-lysine (CML) is an advanced glycation end product (AGE), the accumulation of which has been implicated in the etiology of diabetes complications. Skeletal muscle in diabetes demonstrates altered function, and increased accumulation of CML has been found in several fast-twitch muscles of diabetic animals. OBJECTIVE: This study aims to explore the accumulation of CML in soleus (a slow muscle) in diabetic animals, with and without insulin therapy. METHODS: Twenty-one rats were randomly divided into control and diabetes groups (DNI: diabetes without insulin; DI: diabetes with insulin; C: control). Diabetes was induced by intravenous administration of streptozotocin. At the end of the 12-week experimental period the soleus muscle was excised and snap frozen in liquid nitrogen. Muscle cross-sections were immunolabeled for CML. The number of CML-labeled muscle fibers was quantified; fibers were also evaluated for fiber types and cross-sectional areas. RESULTS: The percentage of myofibers immunolabeling for CML was highest in the DNI group (13.8 +/- 2.5%), lower in the DI group (5.4 +/- 1.1%) and lowest in the C group (2.1 +/- 0.6%). Statistical analysis revealed that AGE accumulation was significantly greater in the DNI group than in both C and DI groups (p = 0.0002). There was no significant difference between C and DI groups. In the DNI animals, AGE-positive myofibers showed a higher percentage of fast fiber types than did the AGE-negative fibers (49.5 +/- 6.9 vs. 13.7 +/- 1.5%, p = 0.002). No differences existed in cross-sectional areas between AGE-positive and AGE-negative fibers within any group. CONCLUSION: The greatest accumulation of AGE was in the soleus of the DNI group, and was significantly less in the DI group. These findings may be linked to disordered glucose metabolism, increased oxidative stress and/or fiber type transformation in these muscles.
机译：背景：Nepsilon-（羧甲基）-赖氨酸（CML）是晚期糖基化终产物（AGE），其积累已与糖尿病并发症的病因有关。糖尿病患者的骨骼肌表现出功能改变，并且在糖尿病动物的几条快肌中发现了CML的积累增加。目的：本研究旨在探讨接受和不接受胰岛素治疗的糖尿病动物比目鱼肌（慢肌）中CML的积累。方法：21只大鼠随机分为对照组和糖尿病组（DNI：无胰岛素的糖尿病; DI：有胰岛素的糖尿病; C：对照组）。通过静脉内施用链脲佐菌素诱导糖尿病。在12周的实验期结束时，将比目鱼肌切除并在液氮中速冻。用CML免疫标记肌肉横截面。定量CML标记的肌肉纤维的数量;还评估了纤维的纤维类型和横截面积。结果：CNI肌纤维免疫标记的百分比在DNI组最高（13.8 +/- 2.5％），在DI组较低（5.4 +/- 1.1％），而在C组最低（2.1 +/- 0.6％） ）。统计分析表明，DNI组的AGE积累明显高于C组和DI组（p = 0.0002）。 C组和DI组之间没有显着差异。在DNI动物中，AGE阳性肌纤维显示出比AGE阴性纤维更高的快纤维类型百分比（49.5 +/- 6.9与13.7 +/- 1.5％，p = 0.002）。在任何组中，AGE阳性纤维和AGE阴性纤维之间的横截面积均无差异。结论：AGE的最大积累是在DNI组的比目鱼肌中，而DI组则明显更少。这些发现可能与这些肌肉中葡萄糖代谢紊乱，氧化应激增加和/或纤维类型转化有关。

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