RUNX3 inhibits cell proliferation and induces apoptosis by TGF-beta-dependent and -independent mechanisms in human colon carcinoma cells.

Tong DD; Jiang Y; Li M

首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >RUNX3 inhibits cell proliferation and induces apoptosis by TGF-beta-dependent and -independent mechanisms in human colon carcinoma cells.

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RUNX3 inhibits cell proliferation and induces apoptosis by TGF-beta-dependent and -independent mechanisms in human colon carcinoma cells.

机译：RUNX3通过TGF-β依赖性和非依赖性机制在人结肠癌细胞中抑制细胞增殖并诱导凋亡。

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BACKGROUND: Genes involved in the TGF-beta signaling pathway are often altered in several types of cancers. The TGF-beta-resistant human colon cancer cell line HT-29 has inactivated TbetaRII and deficient expression of RUNX3 and Smad4, which are involved in the TGF-beta signaling pathway. METHODS: Western blot and immunocytochemistry were performed to confirm gene expression, the MTT assay to detect cell growth, flow cytometry to investigate the cell cycle and the TUNEL to detect cell apoptosis. RESULTS: In the absence of TGF-beta, Bim was upregulated, cell growth was inhibited and apoptosis was induced. TGF-beta treatment did not affect RUNX3 expression; however, the increase in Bim expression was significant and time dependent. Interestingly, Smad4 but not Smad2/3 was also upregulated upon exposure to TGF-beta. This was not the case after TGF-beta treatment of parent HT-29 cells. As expected, TGF-beta further inhibited cell growth and induced apoptosis in HT-29/RUNX3+ cells. CONCLUSION: Our data demonstrate that RUNX3 is involved in TGF-beta-dependent and -independent cell growth inhibition and apoptosis induction pathways.

机译：背景：参与TGF-β信号通路的基因在几种类型的癌症中经常发生改变。 TGF-beta耐药的人类结肠癌细胞系HT-29已失活TbetaRII以及RUNX3和Smad4的表达不足，这与TGF-beta信号通路有关。方法：采用Western blot和免疫细胞化学法确认基因表达，MTT法检测细胞生长，流式细胞术检测细胞周期，TUNEL法检测细胞凋亡。结果：在没有TGF-β的情况下，Bim被上调，细胞生长受到抑制并诱导了细胞凋亡。 TGF-β处理不影响RUNX3的表达;然而，Bim表达的增加是显着的并且是时间依赖性的。有趣的是，Smad4而不是Smad2 / 3在暴露于TGF-beta后也被上调。 TGF-β处理母体HT-29细胞后情况并非如此。如预期的那样，TGF-β进一步抑制了HT-29 / RUNX3 +细胞的细胞生长并诱导了细胞凋亡。结论：我们的数据表明RUNX3参与了TGF-beta依赖性和非依赖性的细胞生长抑制和凋亡诱导途径。

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《Pathobiology: journal of immunopathology, molecular and cellular biology》 |2009年第4期|共7页
作者
Tong DD; Jiang Y; Li M;
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正文语种 eng
中图分类病理学;
关键词
Transforming Growth Factor beta; Apoptosis; Cell Growth; Hypertension; Human;

机译：转化生长因子β;凋亡;细胞生长;高血压;人类;

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1. RUNX3 inhibits cell proliferation and induces apoptosis by TGF-beta-dependent and -independent mechanisms in human colon carcinoma cells. [J] . Tong DD, Jiang Y, Li M Pathobiology: journal of immunopathology, molecular and cellular biology . 2009,第4期

机译：RUNX3通过TGF-β依赖性和非依赖性机制在人结肠癌细胞中抑制细胞增殖并诱导凋亡。
2. Curcumin inhibits cell proliferation by interfering with the cell cycle and inducing apoptosis in colon carcinoma cells. [J] . Chen H, Zhang ZS, Zhang YL, Anticancer Research: International Journal of Cancer Research and Treatment . 1999,第5Appa期

机译：姜黄素通过干扰结肠癌细胞的细胞周期并诱导其凋亡来抑制细胞增殖。
3. RUNX3 inhibits cell proliferation and induces apoptosis by reinstating transforming growth factor beta responsiveness in esophageal adenocarcinoma cells. [J] . Torquati A, Orear L, Longobardi L, Surgery . 2004,第2期

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4. The Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor ZD1839 (Iressa) Suppresses Proliferation and Invasion of Human Oral Squamous Carcinoma Cells via p53 Independent and MMP, uPAR Dependent Mechanism [C] . EUN JU LEE, JIN HA WHANG, NAM KYEONG JEON, Meeting on Cell Signaling World: Signal Transduction Pathways as Therapeutic Targets . 2007

机译：表皮生长因子受体酪氨酸激酶抑制剂ZD1839（Iressa）通过独立于p53和MMP，uPAR依赖性机制抑制人口腔鳞状细胞癌细胞的增殖和侵袭
5. Reactive oxygen species-dependent mechanisms of N-(4-hydroxyphenyl)retinamide (4HPR)-induced apoptosis in human carcinoma cells. [D] . Kadara, Humam. 2008

机译：N-（4-羟苯基）视黄酰胺（4HPR）诱导的人类癌细胞凋亡的活性氧依赖机制。
6. Mitochondria-dependent and -independent mechanisms in tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis are both regulated by interferon-gamma in human breast tumour cells. [O] . Carmen Ruiz-Ruiz, Abelardo López-Rivas 2002

机译：肿瘤坏死因子相关的凋亡诱导配体（TRAIL）诱导的凋亡中线粒体依赖和独立机制均受人乳腺癌细胞中干扰素-γ的调节。
7. RUNX3 Inhibits Cell Proliferation and Induces Apoptosis by TGF-beta;-Dependent and -Independent Mechanisms in Human Colon Carcinoma Cells [O] . Dan-dan Tong, Ying Jiang, Ming Li, 2009

机译：Runx3抑制细胞增殖并通过TGF-β依赖性和依赖性机制诱导人结肠癌细胞的凋亡

RUNX3 inhibits cell proliferation and induces apoptosis by TGF-beta-dependent and -independent mechanisms in human colon carcinoma cells.

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