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首页> 外文期刊>Sabrao Journal of Breeding and Genetics >Embryogenic callus formation from protoplasts of Chinese chive (Allium tuberosum Rottl.)
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Embryogenic callus formation from protoplasts of Chinese chive (Allium tuberosum Rottl.)

机译：韭菜原生质体的胚性愈伤组织形成

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摘要

Embryogenic calli were derived from shoot tips of two cultivars of Chinese chive (Allium tuberosum). These calli were used to establish donor cell suspension cultures from which protoplasts were produced. Cell division and colony formation from protoplasts proved to be best in agarose-solidified BDS medium containing glucose. Protoplasts of cv. Wide Green produced microcalli, but those of cv. Super Green Belt did not. Small colonies excised from agarose beads developed into callus on solidified MS medium supplemented with 1 mg/L 2,4-D, and these calli produced somatic embryos on hormone-free MS medium. However, no transferable shoots or plantlets were produced.
机译：胚性愈伤组织来源于两个韭菜品种的发芽尖端。这些愈伤组织用于建立供体细胞悬浮培养物，由此产生原生质体。在含有葡萄糖的琼脂糖固化的BDS培养基中，原生质体的细胞分裂和集落形成被证明是最好的。简历原生质体。宽绿产生了微型愈伤组织，但简历产生了。超级绿带没有。从琼脂糖珠上切下的小菌落在补充有1 mg / L 2,4-D的凝固MS培养基上发育成愈伤组织，这些愈伤组织在无激素的MS培养基上产生体细胞胚。但是，没有产生可转移的芽或小苗。

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