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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >Estrogen modulates 5-HT(1A) agonist inhibition of lordosis behavior but not binding of ((3)H)-8-OH-DPAT.
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Estrogen modulates 5-HT(1A) agonist inhibition of lordosis behavior but not binding of ((3)H)-8-OH-DPAT.

机译：雌激素调节5-HT（1A）激动剂抑制脊柱前凸行为，但不结合（（3）H）-8-OH-DPAT。

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Previous studies showed that repeated estrogen treatment reduces the ability of the 5-HT(1A) receptor agonist, 8-hydroxy-2(di-n-propylamino) tetralin (8-OH-DPAT), to inhibit lordosis behavior of female rats. The present study evaluated the effects of repeated estrogen treatment on lordosis behavior and 5-HT(1A) receptor binding and coupling to G protein in the hypothalamus-preoptic area using the agonist ligand [3H]-8-OH-DPAT, which binds selectively to G-protein-coupled 5-HT(1A) receptors. Rats were injected twice with 25 or 50 microg of estradiol benzoate (EB) 7 days apart followed by 500 microg of progesterone (P) 48 h after the second EB injection. Controls received a single injection of 25 or 50 microg EB followed 48 h later by 500 microg of P. Four hours after P, 0.15 mg/kg 8-OH-DPAT was injected, and lordosis behavior examined for 30 min. Rats treated twice with EB showed significantly less 8-OH-DPAT inhibition of lordosis behavior than rats receiving a single EB injection. For receptor binding, rats received EB without P treatment. None of the estrogen treatments reduced [3H]-8-OH-DPAT binding density or affinity in the hypothalamus-preoptic area or hippocampus. These studies suggest that estrogen modulates 5-HT(1A) agonist potency without a measurable change in 5-HT(1A) receptor density or coupling to G protein.
机译：先前的研究表明，反复进行雌激素治疗会降低5-HT（1A）受体激动剂8-羟基-2（二-正丙基氨基）四氢化萘（8-OH-DPAT）抑制雌性大鼠脊柱前凸行为的能力。本研究使用激动剂配体[3H] -8-OH-DPAT评估了重复雌激素治疗对脊柱前凸行为和5-HT（1A）受体结合并耦合到下丘脑前视区G蛋白的影响。对G蛋白偶联的5-HT（1A）受体。在第二次EB注射后48小时，大鼠每隔7天注射25或50 g雌二醇苯甲酸酯（EB），然后注射500 microg孕酮（P）。对照组接受25或50微克EB的单次注射，然后48小时后注射500微克的P。P注射后四小时，注射0.15 mg / kg 8-OH-DPAT，并检查驼背行为30分钟。与接受单次EB注射的大鼠相比，用EB治疗两次的大鼠显示出对脊柱前凸行为的8-OH-DPAT抑制作用明显更少。为了受体结合，大鼠接受未经P处理的EB。在下丘脑前视区或海马区，没有一种雌激素治疗能降低[3H] -8-OH-DPAT的结合密度或亲和力。这些研究表明，雌激素调节5-HT（1A）激动剂的效力，而5-HT（1A）受体的密度没有可测量的变化或与G蛋白偶联。

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