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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >Long-term neurobehavioral and histological damage in brain of mice induced by L-cysteine.
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Long-term neurobehavioral and histological damage in brain of mice induced by L-cysteine.

机译：L-半胱氨酸对小鼠大脑的长期神经行为和组织学损害。

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We investigated whether structural central neural damage and long-term neurobehavioral deficits after L-cysteine (L-Cys) administration in mice is caused by hypoglycemia. Neonatal ICR mice were injected subcutaneously with L-Cys (0.5-1.5 mg/g body weight [BW]) or saline (control). Blood glucose was measured. At 50 days of age, mice were introduced individually into an eight-arm maze for evaluation of spatial memory (hippocampal-related behavior). Times for visiting all eight arms and number of entries until completion of the eight-arm visits (maze criteria) were measured. The test was repeated once daily for 5 days. In situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay was used for detection of brain damage. As early as 20 min and up to 2 h postinjection, animals treated with L-Cys doses higher than 1.2 mg/g BW developed hypoglycemia and looked ill. Several animals convulsed. Long-term survivors required more time, in a dose-dependent manner, to assimilate the structure of the maze, and animals treated with L-Cys (1.5 mg/g BW) exhibited TUNEL-positive changes in the hippocampal regions. All these changes were reversible by coadministration of glucose. We conclude that L-Cys injection can cause pronounced hypoglycemia associated with long-term neurobehavioral changes and central neural damage in mice. Since L-Cys is chemically different from the other excitatory amino acids (glutamate and aspartate), the long-reported L-Cys-mediated neurotoxicity may be connected to its hypoglycemic effect.
机译：我们调查了小鼠中L-半胱氨酸（L-Cys）给药后结构性中枢神经损伤和长期神经行为缺陷是否由低血糖引起。新生儿ICR小鼠皮下注射L-Cys（0.5-1.5 mg / g体重[BW]）或生理盐水（对照组）。测量血糖。在50天大时，将小鼠分别引入八臂迷宫中，以评估空间记忆（海马相关行为）。测量了访问所有八支武器的时间和进入八支武器的访问完成之前的进入次数（迷宫标准）。每天重复测试一次，持续5天。原位末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）检测用于检测脑损伤。早在注射后20分钟至最多2小时，接受L-Cys剂量高于1.2 mg / g BW的动​​物就出现了低血糖症，并且看起来很不适。几只动物抽搐。长期幸存者需要更多的时间，以剂量依赖性的方式吸收迷宫的结构，用L-Cys（1.5 mg / g BW）处理的动物在海马区显示出TUNEL阳性变化。通过共同施用葡萄糖，所有这些变化都是可逆的。我们得出的结论是，L-Cys注射可引起小鼠长期神经行为改变和中枢神经损伤相关的明显低血糖。由于L-Cys在化学上不同于其他兴奋性氨基酸（谷氨酸和天冬氨酸），因此长期以来报道的L-Cys介导的神经毒性可能与其降血糖作用有关。

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