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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >Application of long-term microdialysis in circadian rhythm research.
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Application of long-term microdialysis in circadian rhythm research.

机译：长期微透析在昼夜节律研究中的应用。

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摘要

Our laboratory has pioneered long-term microdialysis to monitor pineal melatonin secretion in living animals across multiple circadian cycles. There are numerous advantages of this approach for rhythm analysis: (1) we can precisely define melatonin onset and offset phases; (2) melatonin is a reliable and stable neuroendocrine output of the circadian clock (versus behavioral output which is sensitive to stress or other factors); (3) melatonin measurements can be performed extremely frequently, permitting high temporal resolution (10 min sampling intervals), which allows detection of slight changes in phase; (4) the measurements can be performed for more than four weeks, allowing perturbations of the circadian clock to be followed long-term in the same animals; (5) this is an automated process (microdialysis coupled with on-line HPLC analysis), which increases accuracy and bypasses the labor-intensive and error-prone manual handling of dialysis samples; and (6) our approach allows real-time investigationof circadian rhythm function and permits appropriate timely adjustments of experimental conditions. The longevity of microdialysis probes, the key to the success of this approach, depends at least in part on the methods of the construction and implantation of dialysis probes. In this article, we have detailed the procedures of construction and surgical implantation of microdialysis probes used currently in our laboratory, which are significantly improved from our previous methods.
机译：我们的实验室率先进行了长期微透析，以监测多个昼夜周期中活动物中松果体褪黑激素的分泌。这种方法在节奏分析中有许多优点：（1）我们可以精确地定义褪黑激素的起效阶段和失调阶段; （2）褪黑素是昼夜节律时钟的可靠和稳定的神经内分泌输出（与对压力或其他因素敏感的行为输出相反）; （3）褪黑激素的测量可以非常频繁地进行，从而具有较高的时间分辨率（10分钟采样间隔），从而可以检测到相位的微小变化; （4）可以进行超过四个星期的测量，从而可以长期跟踪同一只动物的昼夜节律。 （5）这是一个自动化过程（微透析与在线HPLC分析相结合），可提高准确性并绕开透析样品的劳动密集型和易于出错的手动处理; （6）我们的方法可以实时研究昼夜节律功能，并可以适当地及时调整实验条件。微透析探针的寿命是该方法成功的关键，至少部分取决于透析探针的构建和植入方法。在本文中，我们详细介绍了当前在我们实验室中使用的微透析探针的构建和手术植入程序，这些程序与我们以前的方法相比有了很大的改进。

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