• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pharmacology, Biochemistry and Behavior >Blockade of CRF1 and CCK2 receptors attenuated the elevated anxiety-like behavior induced by immobilization stress.
【24h】

Blockade of CRF1 and CCK2 receptors attenuated the elevated anxiety-like behavior induced by immobilization stress.

机译：CRF1和CCK2受体的阻滞减弱了固定压力引起的焦虑样行为的升高。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Two highly co-localized neurotransmitters: corticotropin-releasing factor (CRF) and cholecystokinin (CCK), have been implicated in the development of stress-related anxiety disorders. This study was designed to examine the role of CRF1 and CCK2 receptors on the anxiety-like behavior induced by immobilization stress. Our results showed that 30-min immobilization enhanced the anxiety-like behavior in C57BL/6J mice examined in the elevated plus maze (EPM). The combined pretreatment of CR2945 (a CCK2 receptor antagonist) and antalarmin (a CRF1 receptor antagonist) fully blocked this elevated anxiety-like behavior, while the application of CR2945 or antalarmin alone showed only partial effects. The increased expression of CRF1 and CCK2 receptors at protein levels in three anxiety-related brain regions: cortex, hippocampus and hypothalamus, was detected by Western blot. The increased mRNA expression of CCK, CRF, CCK2 and CRF1 receptors was also examined by real-time RT-PCR. Our study demonstrated that the blockade of CRF1 and CCK2 receptors attenuated the elevated anxiety-like behavior induced by immobilization stress, suggestive of the CRF and CCK systems contributing to the development of stress-related anxiety behavior.
机译：两种高度共定位的神经递质：促肾上腺皮质激素释放因子（CRF）和胆囊收缩素（CCK），已与压力相关性焦虑症的发生有关。本研究旨在检查CRF1和CCK2受体在固定压力诱导的焦虑样行为中的作用。我们的结果表明，在高架迷宫（EPM）中检查的30分钟固定化可增强C57BL / 6J小鼠的焦虑样行为。 CR2945（CCK2受体拮抗剂）和antalarmin（CRF1受体拮抗剂）的联合预处理完全阻断了这种升高的焦虑样行为，而单独使用CR2945或antalarmin则仅显示部分作用。通过蛋白质印迹法检测到三个与焦虑相关的大脑区域（皮质，海马和下丘脑）中蛋白质水平的CRF1和CCK2受体表达增加。还通过实时RT-PCR检查了CCK，CRF，CCK2和CRF1受体的mRNA表达增加。我们的研究表明，CRF1和CCK2受体的阻滞减弱了由固定应激引起的焦虑样行为的升高，提示CRF和CCK系统促进了应激相关焦虑行为的发展。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号