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In vitro antiviral activity from Acanthospermum australe on herpesvirus and poliovirus

机译：Acanthospermum australe对疱疹病毒和脊髓灰质炎病毒的体外抗病毒活性

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Context: The Asteraceae family has been of interest to researchers due to the presence of polyphenolic compounds, mainly flavonoids, which demonstrated antiviral activity. Objective: The hydroethanol extract of the aerial parts of Acanthospermum australe (Loefl.) Kuntze (Asteraceae) and its fractions, were evaluated in vitro for their potential cytotoxic and antiviral activity against bovine herpesvirus and human poliovirus. Materials and methods:ThesulforhodamineBcolorimetric assay were used toevaluate the capacity of the hydroethanol extract and fractions to inhibit the lytic activity of herpes and poliovirus in infected cell cultures and their influence on the viability of uninfected cell cultures. Results and discussion: A progressive increase in the antiviral effect against herpesvirus was observed in the course of the purification process of the extract. The hydroethanol extract had a 50% antiviral effective concentration (EC_(50)) at 70 mug/mL and 36 mug/mL for herpes and poliovirus, respectively, and it exhibited no cytotoxicity. The fractions F3 (dichloromethane) and F4 (dichloromethane: ethyl acetate (1:1 v/v)) both showed EC_(50) at 6.25 mug/mL against herpesvirus, and these fractions showed cytotoxic concentrations (CC_(50)) at 12.7 and 11.7 mug/mL, respectively. These fractions had no effect against poliovirus in the concentrations tested. From the bioactive F3, a diterpene lactone (acanthoaustralide-1-O-acetate) was isolated at a concentration of 0.5% and from F4 two flavonoids (quercetin and chrysosplenol D) were isolated at concentrations of 0.14 and 0.24%, respectively. Conclusion: The present study reports for the first time the antiviral activity of extracts and fractions from A. australe aerial parts.
机译：背景：由于多酚类化合物（主要是类黄酮）的存在，其具有抗病毒活性，因此引起了研究者们的兴趣。目的：体外评价刺五加（Aethespermum australe（Loefl。）Kuntze（Asteraceae）地上部分的乙醇提取物及其级分对牛疱疹病毒和人脊髓灰质炎病毒的潜在细胞毒性和抗病毒活性。材料和方法：磺胺丁丹比色法用于评估水乙醇提取物和馏分抑制感染的细胞培养物中疱疹和脊髓灰质炎病毒的溶解活性的能力及其对未感染的细胞培养物活力的影响。结果与讨论：在提取物的纯化过程中，观察到针对疱疹病毒的抗病毒作用逐渐增强。该氢乙醇提取物对疱疹和脊髓灰质炎病毒分别具有70杯/毫升和36杯/毫升的50％抗病毒有效浓度（EC_（50）），并且没有表现出细胞毒性。馏分F3（二氯甲烷）和F4（二氯甲烷：乙酸乙酯（1：1 v / v））均显示针对疱疹病毒的EC_（50）为6.25杯/毫升，这些馏分的细胞毒性浓度为（CC_（50））为12.7和11.7杯/毫升。在所测试的浓度下，这些级分对脊髓灰质炎病毒没有影响。从生物活性的F3中分离出浓度为0.5％的二萜内酯（棘金蛇毒-1-O-乙酸盐），从F4中分离出两种黄酮类化合物（槲皮素和chrysosplenol D），其浓度分别为0.14和0.24％。结论：本研究首次报道了A.australe地上部分提取物和级分的抗病毒活性。

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