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Antidiarrheal activity, chemical and toxicity profile of Berberis aristata

机译：小Ber小dia的止泻活性，化学和毒性特征

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Context: This study evaluated the in vitro and in vivo antidiarrheal activity, oral acute toxicological profile, and developed a chemical fingerprint of Berberis aristata Linn. (Berberidaceae). Materials and methods: The ethanol (by maceration) and aqueous (by Soxhlet) extracts of Berberis aristata bark were used for the study. The study involved the antimicrobial (minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) by micro dilution) and antidiarrheal (castor oil induced diarrhea, charcoal motility) tests. The active principle, berberine was characterized by different spectroscopic and chromatographic techniques.Results: The MIC and MBC (of both extracts) against all strains of Shigella were recorded between 125 to 500 mug/mL and 300 to 600 mug/mL, respectively.The MIC and MBC values of berberine are almost comparable to standard ciprofloxacin. UV and IR spectroscopy along with HPTLC and HPLC studies showed presence of berberine in the extracts. The onset of castor oil induced diarrhea was delayed and number of diarrheal episodes was reduced by both the extracts in a dose dependent manner. Similarly, the length of intestine traveled by the feed was also significantly reduced in the charcoal motility test by both the extracts. LD_(50) of >5000mg/kg body weight was observed for both extracts in the acute oral toxicity studies with Swiss albino mice. Conclusion: The results validate in vivo and in vitro antidiarrheal activity of Berberis aristata extracts and provide its chemical fingerprint.
机译：背景：这项研究评估了体外和体内的止泻活性，口服急性毒理学特征，并开发了小Ber小ber的化学指纹图谱。 （小ber科）。材料和方法：使用小Ber小Ber树皮的乙醇提取物（浸渍）和水提取物（索氏提取物）进行研究。该研究涉及抗微生物（通过微稀释的最低抑菌浓度（MIC）和最低杀菌浓度（MBC））和止泻（蓖麻油引起的腹泻，木炭运动性）测试。小different碱的有效成分通过不同的光谱和色谱技术进行了表征。结果：记录到的所有菌株对志贺氏菌的MIC和MBC（两种提取物）分别在125至500杯/毫升和300至600杯/毫升之间。小ber碱的MIC和MBC值几乎与标准环丙沙星相当。紫外和红外光谱以及HPTLC和HPLC研究表明提取物中存在小presence碱。两种提取物均以剂量依赖性方式延迟了蓖麻油诱发的腹泻的发作，并减少了腹泻发作的次数。同样，两种提取物在木炭运动性试验中，饲料所带入的肠道长度也大大减少。在瑞士白化病小鼠的急性口服毒性研究中，两种提取物的LD_（50）均> 5000mg / kg体重。结论：该结果验证了小Ber提取物的体内和体外止泻活性，并提供了其化学指纹图谱。

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