• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pesticide Research Journal >Method Validation for the Estimation of Organochlorine Insecticides in Potato
【24h】

Method Validation for the Estimation of Organochlorine Insecticides in Potato

机译：马铃薯中有机氯杀虫剂估算方法的验证

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The study was undertaken to validate the method of analysis for nine organochlorine insecticides namely α-HCH, γ-HCH, heptachlorexoepoxide, heptachlorendoepoxide, aldrin, dieldrin, 2,4 DDT, β-endosulfan, endosulfansulphate in potato. The insecticides were extracted in acetone, partitioned into DCM:Hexane (1:1, v/v), cleaned up by adsorption chromatography on glass column (60 cm x 2 cm id) with purified silica gel (60-120 mesh) and eluted with 200 ml of mixture of dichloromethane: acetone (1:1, v/v). The pesticides were estimated by GC equipped with ~(63)Ni electron capture detector (ECD) and BP, column (30 m x 0.025 mm id) packed with 100% dimethylpolysiloxane. The accuracy, precision and method LOQ (limit of quantification) determined via recovery experiments at their respective spiking levels ranged from 0.0018-0.0072 ug/g with the recoveries of ≥ 85%. The limit of detection (LOD) varied from 0.0005-0.0012. The standard deviation ranged from 0.0006 - 0.0018 while the % RSD ranged from 2.74-13.47. Good linearity (at least r~2≥0.99) of the calibration curve was obtained over the range of 0.001 -0.45 μg/ml. The data for detection limits, mean % recovery and % relative standard deviation revealed that method is acceptable for estimation of all the nine insecticides studied in potato.
机译：进行该研究以验证分析马铃薯中9种有机氯杀虫剂的方法，即α-HCH，γ-HCH，七氯环己氧化物，七氯内环氧化物，艾氏剂，狄氏剂，2,4滴滴涕，β-硫丹，硫丹硫酸盐。将杀虫剂在丙酮中萃取，分配到DCM：己烷（1：1，v / v）中，在玻璃柱（60 cm x 2 cm内径）上用纯化硅胶（60-120目）进行吸附色谱法纯化，并洗脱用200ml二氯甲烷：丙酮的混合物（1∶1，v / v）。农药通过配备〜（63）Ni电子捕获检测器（ECD）的GC和装有100％二甲基聚硅氧烷的BP色谱柱（内径30 m x 0.025 mm）进行评估。通过回收实验在各自的加标水平下测定的准确度，精密度和方法LOQ（定量限）范围为0.0018-0.0072 ug / g，回收率≥85％。检测限（LOD）为0.0005-0.0012。标准偏差的范围是0.0006-0.0018，而RSD的百分比范围是2.74-13.47。在0.001 -0.45μg/ ml的范围内获得了良好的校准曲线线性（至少r〜2≥0.99）。检出限，平均回收率％和相对标准偏差％的数据表明，该方法可用于评估马铃薯中研究的所有九种杀虫剂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号