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首页> 外文期刊>Pesticide Research Journal >Selection of Bacillus spp. Isolates against Fire Blight of Apple on the Basis of Sequencing 16S rDNA
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Selection of Bacillus spp. Isolates against Fire Blight of Apple on the Basis of Sequencing 16S rDNA

机译：芽孢杆菌属的选择。根据16S rDNA序列分离苹果的枯萎病

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摘要

Amplification of the 16S rDNA of 21 isolates of Bacillus spp. was done using polymerase chain reaction. Finally the DNA sequence of three broad groups of Bacillus spp. comprising 18 isolates were studied. The DNA sequence of two isolates of Bacillus subtilis strains (BD 170 and AG 704) and one Bacillus pumilus (isolate KS 1) was found for the first time with new accession number. Most of the isolates of Bacillus spp. were highly effective against Erwinia amylovora causing fire blight of apple in mixed culture experiment and under field condition. Among the isolates tested, Bacillus amyloliquefaciens (Gene bank accession no. AY 651023) isolate name FDK 21 showed 99.1% and 100% effectiveness against fire blight in mixed culture experiment and on the blossom of apple under field conditions respectively. Further Talcum and whey powder were used to prepare the formulation of Bacillus amyloliquefaciens isolate name FDK 21, for its use as biocontrol agent for ecofriendly management of fire blight of apple and enhance the production of apple in Europe.
机译：21个芽孢杆菌属菌株的16S rDNA的扩增。使用聚合酶链反应完成。最后，芽孢杆菌属三大类的DNA序列。研究了包括18个分离株的细菌。首次发现了两个枯草芽孢杆菌菌株（BD 170和AG 704）和一个短小芽孢杆菌（菌株KS 1）的DNA序列，其新登录号为。芽孢杆菌属的大多数分离株。在混合培养实验和田间条件下，均能有效地抗解淀粉欧文氏菌引起苹果枯萎病。在测试的分离株中，解淀粉芽孢杆菌（基因库登录号AY 651023）分离株FDK 21在混合培养实验中和在田间条件下对苹果的开花均显示出99.1％和100％的抗枯萎病效力。还使用滑石粉和乳清粉制备解淀粉芽孢杆菌分离株FDK 21的制剂，用作生物防治剂以生态友好地管理苹果的火疫病并提高欧洲的苹果产量。

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