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首页> 外文期刊>Biomaterials >Formation of melanocyte spheroids on the chitosan-coated surface.
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Formation of melanocyte spheroids on the chitosan-coated surface.

机译：在壳聚糖涂层表面形成黑素细胞球体。

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The search for biocompatible materials that can maintain function of melanocytes as the cellular patch is a feasible alternative for use in the autologous melanocyte transplantation for vitiligo. In this study, we demonstrated that the surface of chitosan-coated polystyrene wells supported the growth and phenotype expression of melanocytes. Depending on the seeding density and culture time, melanocytes were monolayered or spheroidal in morphology. At seeding densities above 10x10(3)cells/cm(2), human melanocytes started to aggregate on the surface of chitosan after 2 days in culture. These aggregates grew into compact melanocyte spheroids on day 3 and more melanocyte spheroids were observed when a higher seeding density was used. Cells remained viable in the spheroids and grew into dendritic melanocytes when they were reinoculated on polystyrene wells. Conversely, the time for the formation of melanocyte spheroids needed a longer period at lower seeding density. For example, melanocytes at as low as 1.25x10(3)cells/cm(2) did not aggregate until the 20th day of culture. In order to interpret the phenomenon further, we proposed the formation of melanocyte spheroids on the chitosan is mediated by a balance between two competing forces: the interactions of cell-chitosan and cell-cell.
机译：寻找可以维持黑素细胞作为细胞补丁的生物相容性材料是用于白癜风的自体黑素细胞移植的可行选择。在这项研究中，我们证明了壳聚糖包被的聚苯乙烯孔的表面支持黑素细胞的生长和表型表达。根据播种密度和培养时间，黑素细胞的形态为单层或球形。在播种密度高于10x10（3）cells / cm（2）的情况下，培养2天后，人黑素细胞开始聚集在壳聚糖表面。这些聚集体在第3天长成紧密的黑素细胞球体，当使用更高的接种密度时，观察到更多的黑素细胞球体。当细胞重新接种在聚苯乙烯孔中时，它们在球体中仍然保持活力，并成长为树突状黑素细胞。相反，在较低的播种密度下，形成黑素细胞球体的时间需要更长的时间。例如，低至1.25x10（3）cells / cm（2）的黑素细胞直到培养的第20天才聚集。为了进一步解释该现象，我们提出壳聚糖上黑色素细胞球体的形成是由两种竞争力之间的平衡介导的：细胞-壳聚糖和细胞-细胞的相互作用。

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