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首页> 外文期刊>Biomaterials >Culturing of skin fibroblasts in a thin PLGA-collagen hybrid mesh.
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Culturing of skin fibroblasts in a thin PLGA-collagen hybrid mesh.

机译：在薄PLGA-胶原蛋白混合网中培养皮肤成纤维细胞。

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A thin biodegradable hybrid mesh of synthetic poly(DL-lactic-co-glycolic acid) (PLGA) and naturally derived collagen was used for three-dimensional culture of human skin fibroblasts. The hybrid mesh was constructed by forming web-like collagen microsponges in the openings of a PLGA knitted mesh. The behaviors of the fibroblasts on the hybrid mesh and PLGA knitted mesh were compared. The efficiency of cell seeding was much higher and the cells grew more quickly in the hybrid mesh than in the PLGA mesh. The fibroblasts in the PLGA mesh grew from the peripheral PLGA fibers toward the centers of the openings, while those in the hybrid mesh also grew from the collagen microsponges in the openings of the mesh resulting in a more homogenous growth. The proliferated cells and secreted extracellular matrices were more uniformly distributed in the hybrid mesh than in the PLGA mesh. Histological staining of in vitro cultured fibroblast/mesh implants indicated that the fibroblasts were distributed throughout the hybrid mesh and formed a uniform layer of dermal tissue having almost the same thickness as that of the hybrid mesh. However, the tissue formed in the PLGA mesh was thick adjacent to the PLGA fibers and thin in the center of the openings. Fibroblasts cultured in the hybrid mesh were implanted in the back of nude mouse. Dermal tissues were formed after 2 weeks and became epithelialized after 4 weeks. The results indicate that the web-like collagen microsponges formed in the openings of the PLGA knitted mesh increased the efficiency of cell seeding, improved cell distribution, and therefore facilitated rapid formation of dermal tissue having a uniform thickness. PLGA-collagen hybrid mesh may be useful for skin tissue engineering.
机译：合成聚（DL-乳酸-乙醇酸）（PLGA）和天然来源的胶原蛋白的可生物降解的薄混合网用于人类皮肤成纤维细胞的三维培养。通过在PLGA针织网眼的开口中形成网状胶原微海绵来构建混合网眼。比较了成纤维细胞在混合网和PLGA编织网上的行为。与PLGA网格相比，混合网格中的细胞播种效率要高得多，并且细胞生长更快。 PLGA网孔中的成纤维细胞从周围的PLGA纤维向开口的中心生长，而混合网孔中的成纤维细胞也从网孔中的胶原微海绵生长，从而导致更均匀的生长。增殖细胞和分泌的细胞外基质比PLGA网格更均匀地分布在混合网格中。体外培养的成纤维细胞/网状植入物的组织学染色表明，成纤维细胞分布在整个混合网中，并形成了皮肤组织的均匀层，其厚度几乎与混合网的厚度相同。但是，在PLGA网眼中形成的组织在PLGA纤维附近较厚，在开口中心较薄。在杂交网中培养的成纤维细胞被植入裸鼠的背部。 2周后形成皮肤组织，并在4周后上皮化。结果表明，在PLGA编织网的开口中形成的网状胶原微海绵增加了细胞播种的效率，改善了细胞分布，因此促进了具有均匀厚度的真皮组织的快速形成。 PLGA-胶原蛋白混合网可用于皮肤组织工程。

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