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首页> 外文期刊>Biomaterials >Immobilization of heparin on a silicone surface through a heterobifunctional PEG spacer
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Immobilization of heparin on a silicone surface through a heterobifunctional PEG spacer

机译：通过异双功能PEG间隔基将肝素固定在硅酮表面上

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A novel method of immobilizing heparin on a silicone surface through a heterobifunctional PEG spacer was used yield well defined surfaces with highly active surface immobilized heparin and low non-specific protein adsorption. The heparin surface density achieved using this technique was 0.68 mu g/cm(2). Sessile drop water contact angles showed increased hydrophilicity of the silicone surface after PEG modification and a further decrease in the contact angles following the grafting of heparin. High specificity for ATIII with little fibrinogen adsorption was noted in plasma adsorption studies. This ATIII adsorption was mediated by the heparin layer, since surfaces modified with PEG only did not adsorb significant quantities of AT. The thrombin resistance of the heparin modified surfaces was demonstrably greater as measured by a chromogenic thrombin generation assay. The results suggest that the heterbifunctional PEG linker results in a high density of active heparin on the surfaces. (c) 2005 Elsevier Ltd. All rights reserved.
机译：通过异双功能PEG间隔基将肝素固定在硅酮表面上的新方法可产生具有高活性的表面固定肝素和低非特异性蛋白质吸附的清晰定义的表面。使用此技术获得的肝素表面密度为0.68μg / cm（2）。无滴水滴接触角显示在PEG改性后有机硅表面的亲水性增加，并且在肝素接枝后接触角进一步降低。在血浆吸附研究中发现对ATIII的特异性很高，而纤维蛋白原的吸附很少。这种ATIII吸附是由肝素层介导的，因为用PEG改性的表面仅不吸附大量的AT。如通过生色凝血酶生成测定所测量，肝素修饰的表面的凝血酶抗性明显更大。结果表明，异双功能PEG连接体在表面上产生高密度的活性肝素。 （c）2005 Elsevier Ltd.保留所有权利。

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